AIMS Insulin-like growth factor type I (IGF-I) antisense cellular gene therapy of tumours is based on the following data: rat glioma or hepatoma cells transfected with the vector encoding IGF-I antisense cDNA lose their tumorigenicity and induce a tumour specific immune response involving CD8(+) T cells. Recently, using the IGF-I triple helix approach in studies of tumorigenicity, major histoco...

Introduction Collagen consists of three polypeptide chains that fold into a triple helix. Each natural chain contains many repeats of the sequence: XaaYaaGly, in which a third of the Xaa and Yaa residues are (2S)-proline (Pro). The pucker of a proline ring can be influenced by electronegative substituents, such as the hydroxyl in the naturally occurring residue, (2S,4R)-4-hydroxyproline (Hyp) [...

The collagen triple helix is characterized by the repeating sequence motif Gly-Xaa-Yaa, where Xaa and Yaa are typically proline and (2S,4R)-4-hydroxyproline (4(R)Hyp), respectively. Previous analyses have revealed that H-(Pro-4(R)Hyp-Gly)(10)-OH forms a stable triple helix, whereas H-(4(R)Hyp-Pro-Gly)(10)-OH does not. Several theories have been put forth to explain the importance of proline puc...

Triple helix-forming oligonucleotides covalently linked to psoralen can be specifically cross-linked to both strands of DNA at the triplex-duplex junction following UV irradiation. We have previously shown that a 15mer psoralen-oligonucleotide conjugate forming a triple helix on the promoter of the alpha subunit gene of the interleukin-2 receptor inhibits transcription of reporter plasmids tran...

-1 Programmed ribosomal frameshifting (PRF) in synthesizing the gag-pro precursor polyprotein of Simian retrovirus type-1 (SRV-1) is stimulated by a classical H-type pseudoknot which forms an extended triple helix involving base-base and base-sugar interactions between loop and stem nucleotides. Recently, we showed that mutation of bases involved in triple helix formation affected frameshifting...

Abstract Since the emergence of Triple Helix, expansions to Quadruple, Quintuple, N-tuple helices, and models decomposing higher-order helices into multiple interrelated triple or two-layer have been proposed. Albeit presenting alternative conceptual frameworks these different Helix seem unsuited address internal boundaries institutional spheres university, industry, government. Addressing this...

Pasca Pandemi Covid-19 Pemerintah Indonesia meluncurkan salah satu program yang dikenal dengan sebutan Pemulihan Ekonomi Nasional atau disingkat PEN. Program PEN ini sebuah kegiatan dibuat tujuan untuk mengurangi dampak terhadap perekonomian. Oleh karena itu, UMKM menjadi sektor khusus perhatian pemerintah dalam upaya Nasional. Para pelaku semestinya memaksimalkan penggunaaan memulihkan kondisi...

Triple helix forming oligonucleotides can direct DNA damaging agents at specific sites in an intact double helix. In our study, triple helix formation was demonstrated in a SV40 based shuttle vector treated with psoralen linked to a 22-mer purine rich oligonucleotide. UVA irradiation caused a covalent linkage of the oligonucleotide through the psoralen to the mutational supF marker gene of the ...