Y chromosome microdeletions can cause male infertility and are classified as natural transmission and de novo mutations. To examine the source of these deletions in Chinese men and to provide a theoretical and laboratory basis for genetic counseling, patients from Northeast China with primary male infertility (N = 22) and their fathers were investigated. Karyotype analysis was performed on peri...

Rapid population growth and urban development along waterways and coastal areas have led to decreasing water quality. To examine the effects of upstream anthropogenic activities on microbiological water quality, methods for source-specific testing are required. In this study, molecular assays targeting human enteroviruses (HEV), bovine enteroviruses (BEV), and human adenoviruses (HAdV) were dev...

BACKGROUND Bovine rotavirus (BRV) infection is common in young calves. This viral infection causes acute diarrhea leading to death. Rapid identification of infected calves is essential to control BRV successfully. Therefore development of simple, highly specific, and sensitive detection method for BRV is needed. RESULTS A reverse transcription loop-mediated isothermal amplification (RT-LAMP) ...

The aim of this paper is to introduce and solve the generalized radical cubic functional equation related to quadratic functional equation$$fleft(sqrt[3]{ax^{3}+by^{3}}right)+fleft(sqrt[3]{ax^{3}-by^{3}}right)=2a^{2}f(x)+2b^{2}f(y),;; x,yinmathbb{R},$$for a mapping $f$ from $mathbb{R}$ into a vector space. We also investigate some stability and hyperstability results for...

Accurate assessment of health risks associated with bovine (cattle) fecal pollution requires a reliable host-specific genetic marker and a rapid quantification method. We report the development of quantitative PCR assays for the detection of two recently described bovine feces-specific genetic markers and a method for the enumeration of these markers using a Markov chain Monte Carlo approach. B...

Abstract Sensitive DNA assays are of importance in life science and biomedical engineering, but they heavily dependent on thermal cycling programs or enzyme-assisted schemes, which require the utilization bulky devices costly reagents. To circumvent such requirements, we developed an isothermal enzyme-free sensing method with dual-stage signal amplification ability based coupling use catalytic ...