34 The prefrontal cortex (PFC) has been implicated in the maintenance of task-relevant information 35 during goal-directed behavior. Using a combination of lesions, local inactivation, and 36 optogenetics, we investigated the functional role of the medial prefrontal cortex (mPFC) in mice 37 using a novel operant delayed alternation task. Task difficulty was manipulated by changing the 38 durati...