The use of sodium sulfate instead of ammonium sulfate in the precipitation of globulins makes it possible to determine the proteins of plasma or serum in small quantities of ‘blood. The basis for the selection of sodium sulfate has been discussed in the preceding paper (1). Robertson (2) described a procedure for the determination of blood proteins in small quantities of blood with the aid of t...

Solubilized protein fractions have been obtained from plasma membranes of Ehrlich ascites cells either by extraction with 0.5% Triton X-100 or by extraction with 2% cholate. Partial purification of the solubilized protein fraction has been obtained by utilizing a combination of ammonium sulfate precipitation and column chromatography. Leucine-binding activity has been detected in the Triton X-1...

particulate and gaseous emissions of pollutants from industries and auto-exhausts are responsible for rising discomfort, aggravates respiratory and cardiovascular. in this study, a system for air quality information in tehran city has been developed, using an online web-based geographic information system. this web-based gis application allows users to recognize areas with a potential increase ...

background : doogh is a fermented beverage in which microbial spoilage is possible. benzoic acid and its salt (sodium benzoate) are added to doogh as antimicrobial preservative to control microbial spoilage. however, the use of this preservative has been prohibited in milk and dairy products such as food and drug administrationand institute of standards and industrial research of iran. thus, th...

crystalline arrays of proteinaceous subunits forming surface layers (s-layers) are now recognized as one of the most common outermost cell envelope components of prokaryotic organisms. the surface layer protein of lactobacillus acidophilus atcc4356 is composed of a single species of protein of apparent molecular weight of 43-46 kda. considering the lactobacillus acidophilus atcc4356 having the ...

Food contaminated with fungal pathogens can cause extremely harmful effects to human even when present in low concentrations. Researchers now pay more attention towards rapid DNA extraction for the quick screening, which is highly demanded in diverse research field. Molecular description of many fungal species is identified by different molecular characteristics. Hence, the efficient isolation ...

In a variation of standard DNA extraction methods, Nocardia asteroides was repeatedly exposed to sodium dodecyl sulfate at 60 degrees C for 30 min; each extraction was followed by centrifugation, removal of the nucleic acid-rich supernatant, and suspension of the cell pellet in fresh sodium dodecyl sulfate. The pooled supernatants contained a substantially higher amount of DNA than the first su...