Background: Clinical evidence indicates the diabetes-induced impairment of osteogenesis caused by a decrease in osteoblast activity. Flavonoids can increase the differentiation and mineralization of osteoblasts in a high-glucose state. However, some flavonoids such as luteolin may have the potential to induce cytotoxicity in osteoblast-like cells. This study was performed to investigate whether...

The potential use of the protein denaturant guanidine hydrochloride to inhibit selectively the enzyme activity of serum alkaline phosphatase isoenzymes from liver, bone, intestine, and placenta was investigated. Inhibition of each isoenzyme was shown to be dependent on time and concentration of inhibitor. In the presence of 0-3 mol/l (28.7 g/l) guanidine hydrochloride for 170 seconds 14%, 47%, ...

ABSTRACT. The effect of cord blood cadmium level on placental alkaline phosphatase activity and birth weight in an oil producing area was studied in this research. The aim of the study was to investigate the effect of cord blood cadmium level on alkaline phosphatase activity and if cadmium concentration had effect on birth weight. The results obtained shows no significant difference (P>0.05) in...

background: there are some controversial data on application of progesterone and progesterone plus estrogen at luteal phase. objective: to investigate the effects of different luteal support hormones on the alkaline phosphates (alp) activity in the endometrial epithelium and endometrial thickness during superovulation process for obtaining the optimized endometrial receptivity in animal model. ...

conclusions ehp was able to both reduce ldl-ch and to significantly decrease markers of oxidative stress and lipid peroxidation induced by hyperlipidemia. therefore, this herb, as a new pharmacological component, could be used to reduce certain blood lipids, lipid peroxidation, and aminotransferase markers. materials and methods thirty-two male rats weighting 200 ± 10 g were randomly divided in...

The potential use of the protein denaturant guanidine hydrochloride to inhibit selectively the enzyme activity of serum alkaline phosphatase isoenzymes from liver, bone, intestine, and placenta was investigated. Inhibition of each isoenzyme was shown to be dependent on time and concentration of inhibitor. In the presence of 0.3 mol/l (28.7 g/l) guanidine hydrochloride for 170 seconds 14%, 47%, ...

The alkaline phosphatase activity of a number of chemicaland viral-induced thymic lymphomas from C57BL mice was characterized biochemically according to pH optimum, heat inactivation, the ratio of activity toward two substrates, p-nitrophenyl phosphate and /3-glycerophosphate, and electrophoretic mobility in acrylamide gel. Alkaline phosphatase activity from nor mal liver, spleen, and duodenum ...

A purified commercial preparation of Escherichia coli alkaline phosphatase (EC 3.1.3.1) has been shown to dephosphorylate several phosphoproteins including bovine heart glycogen synthase D, mixed phosphohistones, and rabbit skeletal muscle phosphorylase kinase but not rabbit skeletal muscle glycogen phosphorylase. Alkaline phosphatase completely removed phosphate groups previously added during ...

abstract the addition of manures and chemical fertilizers as soil amendments for the improvement of soil fertility and quality could affect the seasonal changes in the activity of soil microbes and enzymes. this study aimed at investigating the effect of different levels of nitrogen from broiler litter and urea sources on the trend of urease, alkaline phosphatase and saccharase activities in a ...

Fluorescent probe 1, showing a high fluorescence turn-on signal ratio, enables the real-time imaging of endogenous alkaline phosphatase (ALP) activity in living cells, and the fast and quantitative analysis of enzyme activity at the single-cell level.