Microorganisms producing alkaline phosphatases are wide spread in nature. Alkaline phosphatase (Orthophosphoric monoester phosphohydrolase, E.C.3.1.3.1) is a hydrolase enzyme functioning at alkaline pH. The metalloenzyme containing the two zinc ions are involved in catalysis whereas the magnesium ion is important in structural stabilization (Simpson et al., 1968; Anderson et al., 1975). The enz...

We determined plasma (serum alkaline phosphatase activity in 854 healthy students of the Rochester, Minnesota, public schools. Prepubertal girls had somewhat greater upper limits than did boys, and there was a low trend of increasing activity in both sexes. At the beginning of adolescence increasing activities were observed, which peaked at ages 11 to 12 years in girls and at ages 13 to 14 in b...

Since serum alkaline phosphatase increases in fluoride therapy for osteoporosis, it is generally accepted that fluoride stimulates bone formation. However, histochemical studies have shown that alkaline phosphatase is also increased in resorbing osteocytes. Fluoride is toxic to metabolically active bone cells, alkaline phosphatase is released, and serum alkaline phosphatase increases. We propos...

Alkaline etching of crystalline using KOH (potassium hydroxide), TMAH (tetramethyl ammonium hydroxide), or EDP (ethylenediamene pyrocatecol) is performed for creating various feature definition in MEMS (micro electrical mechanical structures, solar cells, and integrated circuit manufacturing. KOH will be discussed in this section. The alkaline chemistries have the ability to preferentially etch...

105 Abstract: Origin of A-type alkali feldspar granites is currently the subject of a world-wide debate. Contrasting hypotheses have been proposed, which range from an entirely crustal origin to an almost complete mantle derivation. A-type alkali feldspar granites belong to either unimodal granite (rhyolite)-dominated association, or bimodal gabbro (basalt)-granite (rhyolite) suite. It is argue...

An automated method is described for determining alkaline phosphatase activity in serum, with use of sodium thymolphthalein monophosphate as the substrate. The system makes use of standard AutoAnalyzer components, has a simple flow diagram, and does not involve dialysis. The method has good precision, and the results correlate well with those from the manual method of which it is an adaptation.

Purified chicken intestinal alkaline phosphatase is active at pH 8 to 9, but becomes rapidly inactivated with change of pH to 6 or less. Also, a solution of the inactivated enzyme at pH 4.5 rapidly regains its activity at pH 8. In the range of pH 6 to 8 a solution of purified alkaline phosphatase consists of a mixture of active and inactive enzyme in equilibrium with each other. The rate of ina...

inor i8obutanol. Removal of the lipid is accompanied by simultaneous release of the enzyme into true solution. No other organic solvent tested was effective in releasing the bound phosphatase, indicating a unique action of butanol. I wish to thank Dr M. Dixon, F.R.S., for his encouraging interest and advice during this work. This work was carried out while holding a Travelling Scholarship award...