The use of novel mass spectrometric techniques, off-line coupled to chromatographic techniques is described in order to obtain molecular mass information of compounds present in complex mixtures of oligosaccharides. Such information could be quite useful in studies where pure and well-defined enzymes are used to study the chemical fine structure of polysaccharides. Post source decay fragmentati...

A particulate enzyme preparation, obtained from sheep submaxillary gland, catalyzed the transfer of iV-acetyl-Dgalactosamine from its uridine diphosphate derivative to certain protein acceptors. The proteins were prepared from sheep submaxillary mucin by enzymatic or chemical treatments that removed the disaccharide residues from the mucin. Only these proteins served as N-acetylgalactosamine ac...

The temperature-dependent hydrolysis and solubility of chitosan in sulfuric acid solutions offer the possibility for chitosan extraction from zygomycetes mycelia and separation from other cellular ingredients with high purity and high recovery. In this study, Rhizomucor pusillus biomass was initially extracted with 0.5 M NaOH at 120 °C for 20 min, leaving an alkali insoluble material (AIM) rich...

An activity that binds preferentially to depurinated DNA and inserts purines into those sites was partially purified from Drosophila melanogaster embryos. The protein has a sedimentation coefficient of 4.9 S and is devoid of AP (apurinic/apyrimidinic) endonuclease activity. Upon incorporation of purines into apurinic DNA, the number of alkali-labile sites decreases, thus establishing the conver...

Alkali-labile portion covalently linked to the 5' ends of Bacillus subtilis short DNA chains, the putative primer RNA for discontinuous DNA synthesis, was isolated and analyzed using a temperature sensitive DNA polymerase I mutant, which accumulates nascent DNA fragments at a restrictive temperature. A novel oligonucleotide structure as well as mono- to triribonucleotide stretches were isolated...

A rapid, reproducible, and sensitive procedure for the determination of phosphate in several biologic materials is described Organic phosphate is rapidly converted to orthophosphate by digestion with a nitric acid-calcium ion mixture. The acid destroys the organic material while the calcium ion prevents loss of phosphate or its conversion to an unmeasurable form. Inorganic phosphate can be dete...

Abstract: Barley (Hordeum vulgare L.) is a globally significant crop and serves as pioneer for improving saline-alkaline soils due to its salt-alkali tolerant properties. However, the response mechanism of barley alkali stress remains unclear. In this study, two genotypes with contrasting performance under were selected: alkali-tolerant SCMS alkali-sensitive QT9919. The morphological physiologi...

Adventures in Clinical Chemistry (1959 Ernst Bischoff Lecture) 0. H. Gaebler 1 Urine Color Test for the i)etectioa of Phenothiazine Compounds Irene S. Forrest and Fred if. Forrest 11 A Rapid Method for the Estimation of Urinary 17-Ketosteroids F. Rappaport, J. Fischl, and N. Pinto 16 Modifications of Two Methods for the Assay of Amylase Michael Somogyi 23 An Assay for Paiitotlienic Acid in Biol...

Control of native enzyme levels is important when optimizing strains for overproduction of heterologous compounds. However, for many central metabolic enzymes, static knockdown results in poor growth and protein expression. We have developed a strategy for dynamically modulating the abundance of native enzymes within the host cell and applied this to a model system for myo-inositol production f...

OUR knowledge of the role of glucose in the metabolism of the heart in the whole animal appears to be restricted to its extraction from circulating blood and its effect on glycogen elevation, despite extensive studies in isolated tissue and organ preparations. In this study the myocardial ''.oritent of high energy phosphate compounds, estimated as acid-labile phosphate, was examined following i...