To provide insight into the catalytic mechanism for the final deprotonation reaction of squalene-hopene cyclase (SHC) from Alicyclobacillus acidocaldarius, mutagenesis experiments were conducted for the following ten residues: Thr41, Glu45, Glu93, Arg127, Trp133, Gln262, Pro263, Tyr267, Phe434 and Phe437. An X-ray analysis of SHC has revealed that two types of water molecules ("front water" and...

Figure 1: GeneDisc Plate for TAB Spoilage Alicyclobacilli are thermophilic, acidophilic and spore-forming bacteria (TAB) capable of surviving extreme conditions during the fruit juice manufacturing and bottling processes. These spoilage organisms can generate strong off-flavors and off-odors, which negatively affect product quality in fruit juices, concentrates and ingredients used in beverage ...

Using structural insight, the binding mode of isofagomine-derived inhibitors with family GH9 glycosidases is achieved via the study of Alicyclobacillus acidocaldarius (AaCel9A) endoglucanase. In contrast to what was observed in the first report using these compounds with inverting glycosidases from family GH6, these inhibitors do not adopt a distorted conformation in the active site.

Introduction Soft drinks are subject to spoilage induced by bacterial spores resistant to high-temperature. To date, no effective sterilization method that also does not harm the taste profile of the final product has been developed. Filters with pore sizes slightly larger than conventional 0.2μm sterilizing-grade filters were evaluated for their ability to remove heat-resistant bacterial spore...