Six agglutination tests for detecting Toxoplasma gondii-specific antibodies (immunoglobulin G or M) in serum were performed and compared. In total, 599 sera were examined using direct and indirect agglutination assays. Sensitivity varied from 93.7% to 100% and specificity from 97.1% to 99.2%. In a selected population with interfering diseases, the percentage of false positives ranged from 4.3% ...

The mycobacterial agglutination-inhibition test (MAIT) is described as a modification of the mycobacterial agglutination of Schaefer (MATS). It is an organized assemblage of absorption and agglutination tests which is especially useful in the identification of Mycobacterium avium isolates normally excluded from serological identification because of unstable cell suspension characteristics. The ...

In a previous study, we found that some O serotypes of Serratia marcescens, as defined by agglutination tests, were not based on lipopolysaccharide (LPS) O antigens. We developed a dot enzyme immunoassay with a high degree of LPS specificity and tested 104 distinct clinical strains. Only 7 of the 24 existing O antigens were found in more than one strain: O12/O14 (30.8% of strains examined), O21...

Brucellosis is a complicated disease in terms of epidemiology, diagnosis and control. Although many serological tests and new automated blood culture techniques have been developed to diagnose brucellosis, there are still significant problems in the diagnosis of the disease. The present work aimed to evaluate the diagnostic yield of the two main serological tests used for diagnosis of brucellos...

AIM The aim was to study the seroprevalence and efficacy of the different serological tests used for detection of antibody against Brucella species in small ruminants of Banaskantha district of North-Gujarat. MATERIALS AND METHODS Total 1000 serum samples comprising of 485 from sheep and 515 from goat tested for detection of antibodies against the Brucella species by three different serologic...

AIM Brucellosis is a disease of zoonotic importance as it affects both human as well as animal's health, and therefore, directly affects animal productivity and human efficiency. Therefore, a study was conducted to estimate the seroprevalence of brucellosis in humans in Jammu and surrounding areas. MATERIALS AND METHODS A total of 121 sera samples from humans occupied with professional relate...

Representative type strains as well as field strains of Pasteurella multocida from fowl cholera were examined by the indirect hemagglutination procedure and by a rapid plate agglutination test. The former method identified the specific capsular or K substance, and the latter identified the somatic antigens. Inagglutinable organisms were made agglutinable for the rapid procedure by treatment wit...

The first Canadian case of Legionella pneumophila serogroup 12 infection is reported. The initial diagnosis was made with a urine specimen by a broad-spectrum enzyme-linked immunosorbent assay; further serogrouping was done by indirect fluorescent-antibody and tube agglutination tests. This case emphasizes the importance of multivalent urinary antigen detection for the diagnosis of legionellosis.

Brucellosis is the lion's share of infectious disease of animals and it has a particular socio-economic importance for the Republic of Kazakhstan. Sixty percent of epizootic outbreaks of brucellosis identified in the Commonwealth of Independent States (CIS) originated from Kazakhstan in recent years. Definitive diagnosis of brucellosis remains a difficult task. Precisely for this reason, we eva...

Anti-D immunoglobulin; BioVue column agglutination test; Quality control; Haemolytic disease; Anti-Rh(D) titration; Rh-factor Abstract The preparation anti-D immunoglobulin is an important therapy for both treatment and protection. Titer of anti-D immunoglobulin preparation is an important factor to determine the dose of treatment. Many methods are used to evaluate the titer of anti-D immunoglo...