Bloodstream (BSF) and culture forms (CF) of Trypanosoma lewisi were specifically agglutinated with the plant lectins concanavalin A (Con A), soybean agglutinin (SBA), wheat germ agglutinin (WGA), and fucose-binding protein (FBP). Lectin-mediated cell agglutination was inhibited, and reversed in the presence of specific lectin-binding saccharides. Cells were agglutinated randomly with all lectin...

The recent availability of a series of model glycolipids (N-alkylaldobionamides) allowed the systematic investigation of glycolipid-specific agglutination of lipid vesicles by the lectins concanavalin A (Con A) and Ricinus communis agglutinin I (RCA,). The carbohydrate-binding specificities of the lectins determined which glycolipid-containing vesicles were agglutinated and which sugars specifi...

Rapid agglutination of Actinomyces viscosus and Actinomyces naeslundii cells by D-mannose solutions was observed during studies of their attachment to mammalian cells in vitro. The specificity of the agglutination reaction was studied by slide agglutination tests and by measuring the rate of decrease in optical density of bacterial phosphate buffer suspensions caused by the setting of bacterial...

Normal human bone marrow cells were mixed with neuroblastoma cells from four different human cell lines, and the cell mixtures were separated by differential agglutination with soybean agglutinin (SBA). The unagglutinated cell fraction, previously shown to be highly enriched for the hematopoietic pluripotential stem cells and capable of reconstituting lethally irradiated adult patients with acu...

Received: 21 January, 2014 Accepted: 30 March, 2014 Published: 20 April, 2014 A study was conducted to investigate the effect Gonadotropins supplied to culture medium on human sperm parameters and sperm DNA structure during in vitro sperm activation. Thirty infertile patients were included. Semen sample was divided into 3 aliquots. One mL of SMART medium either alone (control group) or supplied...

Addit ion of either 25% or 50% bovine follicular fluid (FF) to Krebs Ringer phosphate buffer, with .5% fructose added (KRPF), maintained higher percentages (P<.05) of motile sperm throughout 9-hr incubation at 37 C than did 100% KRPF or 100% FF. Accelerated sperm moti l i ty and head-to-head agglutination of sperm cells were consistently observed in all diluents that contained FF. The percentag...

Sialic acid is believed to play a critical role in the survival of blood platelets in circulation. Wheat germ agglutinin, which shows specificity for sialic acid, N-acetylglucosamine, and N-acetylgalactosamine, strongly activates platelets. The role of sialic acid in platelet activation by this lectin was studied utilizing neuraminidase-treated platelets and the succinylated lectin that has bee...

Sperm binding activity has been detected in zona pellucida (ZP) glycoproteins and it is generally accepted that this activity resides in the carbohydrate moieties. In the present study we aim to identify some of the specific carbohydrate molecules involved in the bovine sperm-ZP interaction. We performed sperm binding competition assays, in vitro fecundation (IVF) in combination with different ...

Infection is a powerful mechanism that can lead to sperm damage, deformity and eventually, male infertility. Genital tract infection and inflammation have been associated to 8-35% of male infertility cases. E. coli causes agglutination of human spermatozoa and thus, leading to infertility. This study evaluated if the negative influence of Escherichia coli on the motility of human spermatozoa is...