Mastitis is an inflammatory disease condition of udder affecting milk production negatively and having a serious impact on the economy of dairy enterprises. It is considered to be the most costly disease of dairy animals and losses mainly occur through discarded milk, reduction in milk yield, premature culling of animals and replacements. It is usually caused due to the effects of infection by ...

Porcine epidemic diarrhea virus (PEDV), an Alphacoronavirus in the family Coronaviridae, causes acute diarrhea, vomiting, dehydration, and high mortality rates in neonatal piglets. PEDV can also cause diarrhea, agalactia, and abnormal reproductive cycles in pregnant sows. Although PEDV was first identified in Europe, it has resulted in significant economic losses in many Asian swine-raising cou...

The detection of mycoplasma in milk can be performed by either culture techniques or polymerase chain reaction (PCR) based methods. Although PCR can reduce the average diagnostic time to 5 h in comparison with the several days for the isolation of the agent, there is still a need to develop methods, which could give earlier results. For this purpose, we tested the ability of flow cytometry (FC)...

We evaluated the capacity of the Mycoplasma agalactiae p40 gene as a diagnostic marker for contagious agalactia in sheep by quantitative real-time PCR. The p40 gene encodes an immunodominant adhesin that plays a key role in cytoadhesion of M. agalactiae. The assay was 100% specific, with an analytical sensitivity of 1 genome equivalent (GE), a quantification that is highly linear (R(2) > 0.992)...

The first report of disease caused by BoHV1 was made as a venereal disease in a bull and contact cows (Rychner, 1841) which was subsequently referred to as Blaschenausschlag in German literature. The viral aetiology of the disease was proved by Reisinger and Reimann (1928). The first published report on respiratory IBR came from Schroeder and Moys (1954) where they described an apparently new u...

A sudden increase in the incidence of scrapie in Italy in 1997 was subsequently linked to the use of a potentially infected vaccine against contagious agalactia. The relative risk for the exposed farms ranged between 6 and 40. The aim of this study was to assess the long-term impact of exposure to the potentially scrapie-contaminated vaccine on the Italian classical scrapie epidemic. We carried...

Contagious agalactia represents one of the most relevant infectious diseases dairy sheep, with Mycoplasma agalactiae being primary etiological agent. The early, sensitive, and specific identification infected animals, as well development efficient prophylactic tools, remain challenging. Here, we present a comprehensive characterization M. antigens focusing on those shared among different isolat...