We conducted this study to investigate the efficacy of herbal essential oils from 12 species of Zingiberaceae plants to determine their larvicidal and pupicidal activity against fourth instar larvae and pupae of Aedes aegypti and Culex quinquefasciatus mosquitoes. Probit analysis was used to analyze the data. Larval mortality was recorded at 1, 5, 10, 15, 30 and 60 minutes and 24 hours. Pupal m...

INTRODUCTION The mosquito Aedes aegypti is the principal vector of dengue and yellow fever flaviviruses. Temephos is an organophosphate insecticide used globally to suppress Ae. aegypti larval populations but resistance has evolved in many locations. METHODOLOGY/PRINCIPAL FINDINGS Quantitative Trait Loci (QTL) controlling temephos survival in Ae. aegypti larvae were mapped in a pair of F3 adv...

Despite its vector importance little attention is given to Aedes aegypti embryonic development. In this study, temperature influence on time course of Ae. aegypti larvae hatching and egg viability were evaluated. The dormancy state at the end of embryogenesis could be interrupted with a proper stimulus. Temperatures tested ranged between 12-36 degrees C; the maximum temperature limit is 35 degr...

We conducted mosquito surveillance at outdoor breeding habitat in 459 households at 7 urban locations in Putrajaya, Malaysia from January to December 2010 to determine the predominant species and breeding locations. The most common species found at all locations was Aedes albopictus. Gardening utensils were the most common breeding sites. Of the 1,885 mosquito larvae specimens found, 1,774 (94....

Aedes aegypti and Ae. albopictus are the two species of dengue vector mosquitoes in Sri Lanka. Indoor outdoor distribution these species, their population dynamics breeding preference important factors determining transmission. Gampaha district Lanka reported to have frequent epidemics from recent past, hence this study was conducted. Ovitrap surveillance conducted determine indoor Kelaniya Med...

Present study was undertaken to evaluate the suitability of natural (Culex quinquefasciatus) and experimental (Aedes aegypti) vectors for supporting the development of W. bancrofti larvae for onward transmission. Both the species permitted development of W. bancrofti mf to infective larvae (L3) within 11 to 13 days. The mf intake by both the species of mosquitoes was directly related to mf dens...

Mosquito midgut plays a crucial role in its vector susceptibility and pathogen interaction. Identification of the sustainable microflora of the midgut environment can therefore help in evaluating its contribution in mosquito-pathogen interaction and in turn vector competence. To understand the bacterial diversity in the midgut of Aedes aegypti mosquitoes, we conducted a screening study of the g...

Fish as natural predators of mosquito larvae is a way controlling biologically that does not pose risk to the health organisms and environmental damage overcoming problem resistance chemical larvacide. One fish tested in study was rosy barb (Pethia conchonius) lemon cichlid (Labidochromis caeruleus). Objectives: To analyzed difference predation ability male female larvae. Methods: This an exper...

PCR based molecular markers are powerful tools for the analysis of genetic diversity for which isolation of good quality genomic DNA is essential. This paper presents a comparison of three DNA extraction methods for 1, 5 and 10 larvae of Aedes aegypti. First method employing DNAzol® involves simple and one step protocol for DNA isolation where maximum purity (A260/A280 1.9) was obtained with si...

INTRODUCTION In vitro bioassays were performed to access the larvicidal activity of crude extracts from the endophytic fungus Pestalotiopsis virgulata (Melanconiales, Amphisphaeriaceae) and the saprophytic fungus Pycnoporus sanguineus (Basidiomycetes, Polyporaceae) against the mosquitoes Aedes aegypti and Anopheles nuneztovari. METHODS The extracts were tested at concentrations of 100, 200, 3...