Pueraria candollei Wall. ex Benth. var. mirifica (Airy Shaw & Suvat.) Niyomdham was investigated for callus induction using Murashige and Skoog (MS) medium containing different plant growth regulators. After 8 weeks of culture, 66-100% of leaf or stem explants formed calli. Calli from stem explants cultured on MS medium supplemented with 0.5 mg/l thidiazuron (TDZ) gave the maximum of shoot indu...

The present study is the first report on quantitative analysis of secondary metabolites in callus cultures Barleria prionitis L. and comparison with mother plant. Callus was obtained from stem internode (on MS medium 0.5 mg l−1 NAA, BAP 300 ascorbic acid), leaf 1.0 2,4-D, BAP, acid ) explants and, multiplied by subculturing thrice at an interval 25 days. Calli plant counterparts were extracted ...

Excision of Phaseolus vulgaris L. c.v. Red Kidney abscission zone explants results in senescence, mobilization, and abscission. Because these processes take place at about the same time, there has been some question as to whether they are causally related or are occurring in an independent but simultaneous fashion. Data presented here suggest that the latter interpretation is correct. After abs...

From 8-10 days old seedlings of Pisum sativum L. (variety AP-1), various parts root, cotyledon as well as leaf were separated to use as explants. Root and cotyledonary explants did not show any response on MS (Murashige & Skoog) media supplemented with 2, 4-D at a concentration of 5 ppm. However, leaf explants showed callus formation on this media. The callus so formed was subcultured on MS med...

The effect of growth regulators and culture conditions on the morphogenetic response of cotyledonary leaf discs was studied in popular cucumber variety (Cucumis sativus cv. Sheetal). Organogenesis was induced directly without any intervening callus phase on Murashige and Skoog medium supplemented with different concentrations of benzyladenine and indole propionic acid. Best results (93%) were o...

Plants are the important sources of drugs. Secondary metabolites are responsible for therapeutic propertiesin plants. Three compounds including (-)-hyoscyamine, its racemate atropine, and scopolamine (hyoscine) are the most famous tropane alkaloids in the Solanaceae familly. Nowadays, attempts to developthese alkaloids in biotechnological procedures which are principally based on the hairy root...

four separate experiments were conducted to study the effects of factors controlling the establishment of explants from two walnut genotypes z60 and z63 (damavand). factors were explant type, sampling time, explant orientation on the growth medium and antioxidants type. the explants were collected from the collection garden of tooyserkan walnuts research station. four explant types (apical meri...

When abscission in leaf explants from Phaseolus vulgaris, cultivar Red Kidney, was allowed to proceed while the explants were in (2)H(2)O, a 1.25% increase in the buoyant density of cellulase in a cesium chloride gradient was observed. These data indicate that the increase in cellulase activity during abscission is a result of the synthesis of new protein. Two differentially soluble forms of ce...

Plant growth regulators (PGRs) have an important role in callus induction and organogenesis of plant explants cultured in vitro conditions. Callus has a proper potential for plant regeneration through indirect organogenesis and embryogenesis as well suspension culture, genetic transformation and production of secondary metabolites. In current experiment, leaf explants of Petunia × hybrida F1 we...