PPARgamma, a member of the peroxisome proliferator-activated receptor family, is overexpressed in prostate cancer. Natural and synthetic ligands of PPARgamma via genomic and nongenomic actions promote cell cycle arrest and apoptosis of several prostate cancer cells, in vitro. Insulin-like growth factor 1 (IGF-1) inhibits the adriamycin-induced apoptosis of PC-3 human prostate cancer cells. Ther...

BACKGROUND The clinical usefulness of adriamycin is restricted by the development of congestive heart failure. It has been suggested that probucol, a strong antioxidant, completely prevents adriamycin-induced cardiomyopathy without interfering with its antitumor properties. The present study investigated the effects of adriamycin and probucol on myocardial antioxidant enzyme activities and immu...

We have successfully transferred and cloned a fragment of a human multidrug-resistant gene by using DNA-mediated gene transfer. Macromolecular DNA of human multidrug-resistant K562 cells was transfected to drug-sensitive mouse Ltk- cells to obtain a drug-resistant transfectant with a human resistant gene. Both primary and secondary transfectants showed similar patterns of cross-resistance to Ad...

INTRODUCTION. Adriamycin is among the most promising of the currently available anti-cancer drugs (1). While very effective as an anti-tumor agent, it is also extremely toxic. The tumor-inhibiting properties have been shown to result from intercalation of adriamycin with DNA, inhibiting normal enzymatic "unwinding" of the DNA molecule (2). Cardiotoxicity and skeletal toxicity of adriamycin are ...

In the previous study, we generated a rat model of dilated cardiomyopathy (DCM) induced by adriamycin and found that the expression of lncRNA H19 was significantly upregulated in myocardial tissue. The present study was aimed to investigate the potential role of H19 in the pathogenesis of adriamycin-induced DCM. H19 knockdown in the myocardium of DCM rats attenuated cardiomyocyte apoptosis and ...

Flow cytometry has been used to study the influence of Adriamycin- and Corynebacterium parvum-elicited cytostatic macrophages on the cell cycle of HeLa cells. Macrophages harvested from the peritoneal cavity of mice given i.p. injections of Adriamycin retain cytostatic activity for 3 to 4 days in vivo. Many of these macrophages fluoresced with the red emission spectra characteristic of Adriamyc...

Adriamycin is an anthracycline drug with a wide spectrum of clinical antineoplastic activity. However, the usefulness of the drug is limited by its dose-dependent cardiotoxicity. Adriamycin-stimulated free radical formation has been suggested as one of the mechanisms for its cardiotoxic effects. In order to evaluate this underlying mechanism, we have perfused rat hearts with Adriamycin, using a...

BACKGROUND To identify novel proteins involved in multidrug resistance in chronic myeloid leukemia (CML). MATERIALS AND METHODS Comparative proteomics was used to screen multidrug resistance-related proteins from K562 and K562/A02; the differently expressed proteins were further confirmed by western blot and real-time PCR. short hairpin RNA (shRNA) assay was applied to determine the relations...

Adriamycin, a new antitumor antibiotic in the anthracycline group, promptly inhibits DNA and RNA synthesis and arrests cell division. The cell viability (defined as the capacity of a single cell to grow out into a macroscopic clone) is reduced sharply following exposure to adriamycin, 0.1 fig/ml, for a fractional period of the generation time. With the use of a synchronous population of HeLa ce...

objective(s) this study was carried out in order to evaluate the effects of electrochemotherapy, electrical pulses and chemotherapeutic drugs on the killing of cancerous cells and their probable synergistic effects. materials and methods electrochemotherapy treatments conducted on mcf-7 cell line derived from human breast adenocarcinoma tumor using four chemotherapeutic drugs including bleomyci...