Eaton, Philip, Jian-Mei Li, David J. Hearse, and Michael J. Shattock. Formation of 4-hydroxy-2-nonenalmodified proteins in ischemic rat heart. Am. J. Physiol. 276 (Heart Circ. Physiol. 45): H935–H943, 1999.—4-Hydroxy-2nonenal (HNE) is a major lipid peroxidation product formed during oxidative stress. Because of its reactivity with nucleophilic compounds, particularly metabolites and proteins co...

Polycyclic aromatic hydrocarbons (PAHs) can occur in relatively high concentrations in the air, and many PAHs are known or suspected carcinogens. In order to better understand differences in carcinogenic potency between PAHs, we investigated modulation of gene expression in human HepG2 cells after 6 h incubation with varying doses of benzo[a]pyrene (B[a]P), benzo[b]fluoranthene (B[b]F), fluoran...

Adducts of catechols and histidine, which are produced by reactions of 1,2-quinones and p-quinone methides with histidyl residues in proteins incorporated into the insect exoskeleton, were characterized using electrospray ionization mass spectrometry (ESMS), tandem electrospray mass spectrometry (ESMS-MS, collision-induced dissociation), and ion trap mass spectrometry (ITMS). Compounds examined...

2-Amino-3-methylimidazo[4,5-fjquinollne (IQ) and 2-amino-i-methyl 6-phenylimldazo[4,5-bjpyridine (PhIP) are heterocyclic amine mutagens/ carcinogens formed from the cooking of meat. Here we used the pSPi89 shuttle vector developed by Parris and Seidman (Gene, 117: 1—5, 1992) to study and compare the mutation spectra induced by these compounds. pSPi89 was adducted by reaction with the N-acetox...

Selenite (SeL) or selenomethionine (SeM) are the most common selenium (Se) compounds taken as dietary antioxidants to reduce oxidative stress. Because the public may frequently supplement Se compounds at high doses, the possible pro-oxidant effect of Se becomes a concern. SeL and SeM have entirely different pharmacokinetic effects based on dose-related cytotoxicity. Our laboratory has shown pre...

Two compounds known to covalently bind to DNA after their activation with cytochromes P450 (CYPs), carcinogenic benzo(a)pyrene (BaP) and an antineoplastic agent ellipticine, were investigated for their potential to induce CYP and NADPH:CYP reductase (POR) enzymes in rodent livers, the main target organ for DNA adduct formation. Two animal models were used in the study: (i) rats as animals mimic...

The equilibrium constant of adduct formation between some triorganotin chloride of the type R3SnCl (R=C6H5, p-CH3C6H4, n-CH3)CH2)3, C6H5-CH2 and Lewis bases Cl¯, Br¯, and I¯, were measured in 50% v/v ethanol-water solvent spectrophotometrically. The average equilibrium co...

The series of 14 complex organotin(IV) compounds containing many tin atoms and noncovalent bonds in the structure was characterized by electrospray ionization multistage tandem mass spectrometry (ESI-MS(n)). The mass spectra were measured in both polarity modes to obtain complementary structural information. The characteristic pattern of ten natural tin isotopes allowed the determination of the...

The process by which certain classes of toxins compounds or their metabolites may react with DNA to alter the genetic information contained in subsequent generations of cells or organisms are a major component of hazard associated with exposure to chemicals in the environment. Many classes of chemicals may form DNA adducts and there may or may not be a defined mechanism to remove a particular a...