Cationic dye Acridine orange (AO) has wide applications especially in biologicalfields such as analysis of lysosomal and mitochondria content by flow cytometryand so on. In the current work, spectroscopy of acridine orange (AO) dye at bothlow concentrations (mdye/mwater=6.25×10-5, 3.12×10-5) and high concentrations(mdye/mwater=0.002, 0.001) was studied in confined water ...

Energy level alignment and spin polarization at tetracyanoquinodimethane/Fe and acridine orange base/Fe interfaces are investigated by means of photoelectron spectroscopy and X-ray magnetic circular dichroism (XMCD), respectively, to explore their potential application in organic spintronics. Interface dipoles are observed at both hybrid interfaces, and the work function of Fe is increased by 0...

Hepatic proteolysis is inhibited by cell swelling following a variety of experimental manoeuvres, such as reduction of extracellular osmolarity, concentrative uptake of amino acids, or blockade of K+ channels by barium. On the other hand, proteolysis is known to be accomplished by pH-sensitive lysosomal proteases. Accordingly, NH3/NH4+ inhibits proteolysis by intralysosomal alkalinization. The ...

Background/Aim: The aim of this study was to evaluate the cytotoxic and apoptotic effects of chloroform extract (ASCH) from the seeds of Annona squamosa. Materials and Methods: MTT assay was employed to determine the cytotoxicity of the extract on A-549 and EAC cells and IC50 values were calculated. The apoptotic effects induced by the ASCH was evaluated by Acridine Orange Ethidium Bromide dual...

Of 7,871 blood cultures from hospital patients, 22 yielded growth of Streptococcus pneumoniae or Haemophilus influenzae type b. The identities of 19 (86%) of these 22 strains could be verified after 18 to 24 h of incubation by application of the Directigen meningitis test kit to the unheated, uncentrifuged supernatant from the cultures; thus, the turnaround time for these cultures was halved. G...

The sensitivity of three methods for the rapid identification of Cryptococcus neoformans was compared. These were: direct microscopy of india ink preparations, acridine orange staining followed by fluorescence microscopy and detection of cryptococcal capsular polysaccharide antigen by latex agglutination. The overall limit of detection was 3.5 +/- 5.4 X 10(3) CFU/ml (mean +/- SD, n = 27). When ...

The monomer-dimer equilibrium in several ionic dyes (Methylene Blue, Acridine Orange, Nile Blue A, Neutral Red, Rhodamine 6G and Safranine O) has been investigated by means of UV-Vis spectroscopy. The data have been processed by a recently developed method for quantitative analysis of undefined mixtures, based on simultaneous resolution of the overlapping bands in the whole set of absorption sp...