background: ‍‍cryopreservation has some detrimental impacts on sperms surface molecules. modification of the sperm surface molecules can affect on fertility rate. one of the important surface molecules are glycoconjugates. objective: the objective of this study was to evaluate the changes of content of the glycocalyx after standard cryopreservation procedure. materials and methods: forty five h...

The title compound, C(13)H(10)N(+)·C(7)H(4)NO(4) (-), consists of a protonated acridinium cation and a 6-carb-oxy-pyridine-2-carboxyl-ate monoanion. The carboxyl-ate group of the anion appears to be delocalized on the basis of the nearly equivalent C-O bond lengths. In the crystal, the anions are connected by strong O-H⋯O hydrogen bonds, forming chains along the b axis. The acridinium cations a...

AbstractThe purpose of this investigation was to evaluate the antiproliferative and apoptogenic mechanistic studies of methanol extract of Anthocephalus cadamba (MEAC) on Dalton’s lymphoma ascites (DLA) cells treated mice. Determination of antiproliferative activity was performed by using different DLA cells (2×106 cells, i.p.) inoculated mice groups (n=12). Groups were treated for 14 consecuti...

Purpose: To image leukocyte adherence to the vascular endothelium and extravasation into surrounding tissues in neovascularized corneas using acridine orange, a selective DNA stain, in rats Methods: Corneal neovascularization was induced by placing two sutures in the cornea of one eye of 12 Long-Evans rats. One week later, acridine orange (4 mg/kg) was injected via the tail vein and the eyes we...

The histologic evaluation of endochondral ossification is critical to the study of fracture healing, developmental biology, and comparative histology. A modification of Sayers’ alcian blue hematoxylin staining technique is described in which a different counterstain is applied. Paraffin sections are stained in alcian blue hematoxylin for 30 min, and then placed in orange G-eosin counterstain fo...

Acridine orange (AO) has been used to vitally stain somatic and germinal nuclei in vivo (1, 2) and to differentially fluorochrome living and dead bacteria, yeast, and onion root tip cells. The latter phenomenon was discovered by Strugger, whose book, Fluoreszenzmikroskopie und Mikrobiologie (7), details the technics required for adapting the metafluorochromatic staining as a test of cell viabil...

Dechorionated zebrafish embryos were irradiated at 1.5 h post fertilization (hpf) to low-doses of alpha particles, viz., 1.4, 2.8, 5.6, 11.2 mGy (determined using Monte Carlo simulations). At 24 hpf, these embryos were then examined for apoptotic cells through acridine orange staining. The mean number of apoptotic cells was found to decrease significantly from controls to 1.4-mGy irradiation, a...

Acridine orange (AO) has been used to vitally stain somatic and germinal nuclei in vivo (1, 2) and to differentially fluorochrome living and dead bacteria, yeast, and onion root tip cells. The latter phenomenon was discovered by Strugger, whose book, Fluoreszenzmikroskopie und Mikrobiologie (7), details the technics required for adapting the metafluorochromatic staining as a test of cell viabil...

Characteristic features are: the leaf in T/S shows an epidermis of colourless, thickwalled, non-suberised cells, a band of photosynthetic and vascular tissue around the leaf perimeter (2+3), a central core of large thin-walled cells with contents staining pink-orange with KOH solution; yellow-green stomata and idioblasts containing bundles of calcium oxalate raphides (1), each up to 200μ long, ...

The present study was aimed to investigate the photoprotective effect of the male flower of J. regia L. (MEJR) against ultraviolet-B induced apoptosis in human skin cells. Human skin epidermal keratinocytes were pretreated with the MEJR (80 µg/ml, has been selected after MTT assay), prior to 30 min UVB-irradiation at a dose of 20 mJ/cm2. Mitochondrial membrane potential was evaluated using Rhod...