Awareness of the problem of antimicrobial resistance (AMR) has escalated and drug-resistant infections are named among the most urgent problems facing clinicians today. Our experiments here identify a transporter interactome and portray its essential function in acquisition of antimicrobial resistance. By exposing E. coli cells to consecutive increasing concentrations of the fluoroquinolone nor...

conclusions the remarkable rates of biofilm production ability among clinically isolated staphylococci emphasize the necessity of more effective infections control policies to prevent biofilm formation on medical devices and hospital environmental surfaces. background biofilm formation capacity is recognized as an important virulence factor in staphylococci that makes the organisms more resista...

abstract background: the invasion of microorganisms into the bloodstream and spread to different parts of body can cause disruption the functions of vital organs and even death. the proper and prompt treatment is essential and can significantly reduce morbidity and mortality rates. this study aimed to determine the frequency and the antimicrobial susceptibility of bacteria isolated from blood c...

background: recent analyses of hospital outbreaks have documented the spread of resistance to imipenem, which is cur­rently a major problem among gram positive and gram-negative bacteria. the aim of this study was to describe the rate of gram- positive and gram-negative isolates resistance to imipenem as an antibiotic. methods: recorded files of 242 hospitalized patients with at least one sampl...

the objective of this study was to determine the frequency of microorganisms isolated from blood samples of patients admitted to intensive care unit (icu), and the rate of their resistance to common administered antibiotics in an iranian icu. one-hundred patients with systemic inflammatory response syndromes (sirs), hospitalized in icu of sina hospital, tehran, iran, were entered to this cross-...

background and objectives: the emergence of extended-spectrum β-lactamase (esbl)-producing shigella spp.  is of increasing clinical concern specially in children worldwide. the aim of this study was to investigate the occurrence of extended-spectrum β-lactamase producing shigella spp. in tehran, iran. materials and methods: the study included all shigella isolates recovered from pediatric patie...

conclusions periodic evaluation of antimicrobial susceptibility for c. perfringens should be performed. harboring of enterotoxigenic c. perfringens in individuals not necessarily results in diarrhea. results of 136 stool samples including diarrhea [48] and non-diarrhea [88] ones, 83 (61.02%) c. perfringens were cultured. of these 83, 79 c. perfringens isolates showed the alpha-toxin (phospholip...

conclusions: ten percent of the isolates were positive for meca gene among the vancomycin-oxacillin resistant strains, while van gene was not detected in any of the isolates. the data obtained would be useful in clinical control of resistant staphylococcal strains. results: a total of 120 staphylococcus isolates were screened, out of which, 32 (26%) were susceptible to both methicillin and vanc...

BACKGROUND One of the most important resistance mechanisms in bacteria is the increased expression of multidrug efflux pumps. To combat efflux-related resistance, the development of new efflux pump inhibitors is essential. MATERIALS AND METHODS Ten phosphorus ylides were compared based on their MDR-reverting activity in multidrug efflux pump system consisting of the subunits acridine-resistan...

Previously, we found that the quorum sensing transcription factor SdiA up-regulates AcrAB. Others found that a 4-quinolone was a quorum-sensing signal in Pseudomonas aeruginosa. In Escherichia coli, there are at least three multidrug transporters (AcrAB/TolC, MdfA, and NorE) that exude fluoroquinolones. Here, we show that DeltaacrAB, tolC210, or DeltanorE mutants have the same growth rate as WT...