The tolerance and bioremoval of dimethylarsinic acid (DMA(V)) by Acidithiobacillus ferrooxidans (A. ferrooxidans) were investigated here. The inhibitory concentration (IC) of DMA(V) was determined for A. ferrooxidans. The effects of various parameters such as pH, contact time, initial DMA(V) concentration, biosorbent dose and temperature were systematically examined to study the biosorption pro...

The growth yield of Thiobacillus intermedius is greater in glucose-yeast extract or glucose-casein hydrolysate broth than in comparable media without glucose. The quantity of glucose utilized in the glucose-supplemented media is much greater than the increase in cell yield observed relative to the unsupplemented media. Addition of glucose to cell-free extracts of glucose-yeast extract or glucos...

When the strictly autotrophic bacterium Thiobacillus thiooxidans was grown on sulfur, keto acids accumulated in the medium until they reached an inhibitory level at which growth ceased. Much greater growth was possible if these substances were continually dialyzed out of the medium.

Studies of the obligate chemoautotrophic iron bacteria have been largely descriptive in character. This situation is not due to any lack of interest in the physiology and biochemistry of these bacteria, but rather to the inability to grow them in large numbers, and the attendant problem of harvesting cells free from the voluminous precipitate of iron that accompanies growth. Silverman and Lundg...

Eight species of Lycidae are newly recorded from New Brunswick, Canada, bringing the total number of species known from the province to 16. The first documented records from New Brunswick are provided for Greenarius thoracicus (Randall) Erotides scuptilis (Say), and Calopteron terminale (Say) reported by Majka et al. (2011). Eropterus arculus Green, Lopheros crenatus (Germar), and Calochromus p...

A specific and very sensitive dot-immunobinding assay for the detection and enumeration of the bioleaching microorganism Thiobacillus ferrooxidans was developed. Nitrocellulose spotted with samples was incubated with polyclonal antisera against whole T. ferrooxidans cells and then in I-labeled protein A or I-labeled goat antirabbit immunoglobulin G; incubation was followed by autoradiography. S...

It is shown that there exists in the autotrophic bacterium Thiobacillus thiooxidans a measurable oxygen uptake in the absence of the specific nutrient (sulfur). This respiration is shown to be due to the utilization of organic materials which must have been previously synthesized by the chemosynthetic process, providing evidence that autotrophic bacteria contain a dissimilatory process which in...