The extracellular acidic milieu in bones results in activation of osteoclasts (OC) and inhibition of osteoblasts (OB) causing a net loss of calcium from the skeleton and the deterioration of bone microarchitecture. Alkalinization through supplementation with potassium citrate (K citrate) has been proposed to limit the osteopenia progression, even though its pharmacological activity in bone micr...

Advent of pharmacology, combinatorial chemistry and High Through Put Screening offered large number of synthetic drugs as a therapeutic substances. These molecules are potent to elucidate their pharmacological activity. However, pharmacokinetic properties of such molecules are rate limiting factors for its safety as well as efficacy. Most of the synthetic drug available so far in market are eit...

background: induced pluripotent stem cells (ipscs) can be obtained from autologous cells for therapeutic purposes. so, far, many studies have been done to produce induced pluripotent cells by transferring specific pluripotency genes using different methods. in this study, pluripotency gene expression induced by external stresses was assessed in cord blood mononuclear cells. methods: in this exp...

there are two main methods for preparing the puramatrix (pm) as a three-dimensional (3-d) scaffold for supporting the cells including surface plating and encapsulation. in the surface-plating procedure, the scaffold is created by adding an ionic agent such as medium to pm. after adjusting the ph by changing the medium, cells are seeded on top of the scaffold. in the encapsulation method, pm and...

Anticoagulant properties of culture medium conditioned by human umbilical vein endothelial cells were investigated. In the presence of conditioned medium, both prothrombin time and activated partial thromboplastin time of normal plasma were prolonged by 4 and 10 seconds respectively compared with those in the presence of nonconditioned medium. The prologation of coagulation time was not affecte...

Zirconium tungstate as an inorganic ion exchange material has been synthesized at different conditions. The materials were characterized by means infrared spectroscopy, thermo gravimetric analysis, and X-ray diffraction techniques. The stability of the ion exchangers was investigated in acidic and alkaline media. Ion exchange properties of synthesized materials were studied by measuring the dis...

The growth of Candida albicans in RPMI1640 medium was inhibited by sodium sulfite between pH 3-6. Under the condition of pH 7, growth of C. albicans was not inhibited by sodium sulfite. Under an acidic pH condition, sodium sulfite had a candidacidal effect and the activity was expressed within 150 min. The concentration of ATP in C. albicans was decreased by sodium sulfite. Ethanol production b...

The expression of interleukin 8 (IL-8) by human ovarian cancer cells correlates directly with disease progression, but the exact mechanism of IL-8 induction is not clear. The extracellular pH in solid tumors is generally acidic because of elevated acid production and impaired clearance of acidic metabolic wastes. We determined whether acidic conditions also regulate the expression of IL-8 in hu...

The exact role of the central acidic domain of Mdm2 in p53 degradation remains unclear. We therefore performed a systematic and comprehensive analysis of the acidic domain using a series of short deletions and found that only a minor part of the domain was indispensable for Mdm2-mediated p53 ubiquitylation. Moreover, we identified a short stretch of acidic amino acids required for p53 degradati...