................................................................................................................................... iii List of publications .................................................................................................................. iv List of publications not included in this thesis ....................................................................... v...

Cell wall (CW) degradation causes texture loss of plant-based products after processing. However, these losses differ in intensity, which could be due to cell structure or plant tissue internal pH conditions. To distinguish two factors, CWs isolated from apple, beet and kiwifruit were subjected boiling at 2.0, 3.5 6.0. Pectin depolymerization was the least pronounced 3.5, while galacturonic aci...

Acid-catalyzed hydrolysis of the methox-ytropone moiety of colchicine has been shown to proceed at a greater rate than in the case of isocolchicine1. (Struc­ tures are shown in Table I.) No interpretation of this rate difference has been offered. And, while a mecha­ nism for the acid-catalyzed hydrolysis of 2 -methoxytropone has been proposed2, no kinetic data on this type of reaction either fo...

Neisseria gonorrhoeae readily underwent autolysis when suspended in N-2-hydroxyethylpiperazine-N'-2-ethanesulfonic acid (HEPES) buffer at alkaline pH values. Autolysis was inhibited by the addition of Mg2+ or other divalent cations. Autolysis was also suppressed at acid pH (pH 6.0). Suspension of cells in buffer was accompanied by the hydrolysis of peptidoglycan. The rate of peptidoglycan hydro...

A new method was developed to detect sarin hydrolysis products from erythrocytes of four victims of sarin (isopropylmethylphosphonofluoridate) poisoning resulting from the terrorist attack on the Tokyo subway. Sarin-bound acetylcholinesterase (AChE) was solubilized from erythrocyte membranes of sarin victims, digested with trypsin, the sarin hydrolysis products bound to AChE were released by al...

Computational Fluid Dynamic (CFD) modeling of a continuous countercurrent hydrolysis process was performed using ANSYS-CFX. The liquid properties and flow behavior such as density, specific heats, dynamic viscosity, thermal conductivity, and thermal expansivity as well as water solubility of the hydrolysis components triglyceride, diglyceride, monoglyceride, free fatty acid, and glycerol were c...