This study was designed to determine whether methyl-β-cyclodextrin (MCD) can substitute for albumin in incubation medium for neonatal swine adipose tissue explants, or whether MCD affects metabolism and cytokine expression. Subcutaneous adipose tissue explants (100 ± 10 mg) were prepared from 21-day-old pigs. Explants were incubated in medium 199 supplemented with 25 mM HEPES, 1.0 nM insulin at...

LITTLE, JOHN R., MASAI-URO GOTO, AND JOHN J. SPITZER. E$ect of ketones on metabolism of FFA by dog myocurdium and skeletal muscle in Go. Am. J. Physiol. 219(5): 1458-1463. 1970.-The uptake of P-hydroxybutyrate (p-OHB), acetoacetate (AcAc), and free fatty acids (FFA) by the myocardium and the thigh skeIeta1 muscle of mongrel dogs was measured under control conditions and during the infusion of N...

Degree of unsaturation of fatty acids, which is influenced by lipid source and level of metabolism in the rumen, is a major determinant in how dietary lipids affect genes that regulate beef marbling. A total of 28 Red Norte bulls with an initial live weight of 361±32 kg (P>0.05) were used in a completely randomized experimental design to analyze the expression of genes that are involved in lipi...

Environmental factors, especially temperature and light exposure, influence the health and productivity of dairy cows during lactation, possibly via similar physiological mechanisms. For example, heat stress is a critical component of decreased milk yield during summer. However, less is known about the effect of heat stress during the dry period. The objective of this study was to evaluate the ...

BACKGROUND In mammals, the Tribbles family includes widely expressed serine-threonine kinase-like proteins (TRIB1, TRIB2 and TRIB3) that are involved in multiple biological processes including cell proliferation and fatty acid (FA) metabolism. Our recent studies highlighted the importance of FA metabolism in cumulus cells (CC) during oocyte maturation in vertebrates and reported a higher TRIB1 ...

Several studies in dairy cows have shown a relationship between milk fat depression (MFD) and alterations caused in lipogenic gene expression by dietary nutrients. However, information on small ruminants is not only scarce but also inconsistent. Therefore, this experiment was conducted in dairy ewes to study the effect of a diet known to induce MFD on milk fatty acid (FA) composition and mRNA a...

The oxidation of sarcosine (N-methylglycine) by purified rat liver sarcosine dehydrogenase is known to require the participation of a flavin adenine dinucleotide-dependent protein, the electron-transferring flavoprotein, for efficient transfer of electrons between reduced dehydrogenase and 2,6-dichlorophenolindophenol (l), or cytochrome c (a), or cytochrome b (2). On the basis of spectral evide...

The oxidation of sarcosine (N-methylglycine) by purified rat liver sarcosine dehydrogenase is known to require the participation of a flavin adenine dinucleotide-dependent protein, the electron-transferring flavoprotein, for efficient transfer of electrons between reduced dehydrogenase and 2,6-dichlorophenolindophenol (l), or cytochrome c (a), or cytochrome b (2). On the basis of spectral evide...

There are very few studies in ruminants characterizing mammary and adipose tissue (AT) expression of genes and gene networks for diets causing variations in milk fatty acid (FA) composition without altering milk fat secretion, and even less complementing this information with data on tissue FA profiles. This work was conducted in sheep in order to investigate the response of the mammary gland a...

The objective of this study was to evaluate the effects of the different ratios of acetate and β-hydroxybutyrate (BHBA) on cell viability, triacylglycerol (TAG) content, and mRNA expression of the genes related to lipid and protein synthesis in bovine mammary epithelial cells (BMECs). Primary cells were isolated from the mammary glands of Holstein dairy cows and were passaged twice. Then, the c...