BACKGROUND Erythrocyte-magnetized technology (EMT) is a new fully automated method for ABO-RH-K phenotyping and antibody detection. The magnetization of red cells avoids centrifugation and washing phases. This report describes the results of an evaluation of this new technology on its specific automated system. STUDY DESIGN AND METHODS ABO-RH-K phenotyping was compared between EMT and a semia...

A new chemical approach to synthesizing human ABO histo-blood type 2 antigenic determinants was developed. N-Phthaloyl-protected lactosaminyl thioglycoside derived from lactulose via the Heyns rearrangement was employed to obtain a type 2 core disaccharide. Use of this scheme lowered the overall number of reaction steps. Stereoselective construction of the α-galactosaminide/galactoside found in...

PURPOSE ABO incompatible (ABOi) kidney transplantation (KT) has been increasing to compensate for the shortage of organ donors. However, detailed comparative analyses of ABOi KT with ABO compatible (ABOc) KT are still rare. METHODS This retrospective study compared 12 consecutive ABOi KTs to 50 ABOc KTs that employed the same maintenance immunosuppressive agents during the same period. Compar...

Platelets were obtained by differential centrifugation from Group A donors and were incubated with Na2-51Cr04. After washing to remove excess chromium, the platelets were exposed for one hour to Anti A antibody which showed agglutinating activity only. Chromium release was no greater than that of controls. Hemolytic Anti A in the presence of rabbit complement released 15 percent more chromium t...

It is widely accepted that the serum of healthy individuals contains natural antibodies only against those blood group A or B antigens that are not expressed on the individual’s red blood cells. The mechanisms involved in tolerance to autologous blood group antigens remain unclear. In the present study, we show that IgM and IgG antibodies reactive with autologous blood group antigens are presen...

We compared the serological phenotypic frequencies of ABO, MNSs, and Duffy in 417 blood donors and 309 malaria patients from four Brazilian Amazon areas. Our results suggest no correlation between ABO phenotype and malaria infection in all areas studied. We observed significant correlation between the S +s +, S +s -, and S -s + phenotypes and malaria infection in three areas. Some of the Duffy ...

BACKGROUND Grafts survive despite blood group antigens on the transplant being continuously exposed to antibodies in the blood of recipients in ABO-incompatible kidney transplantation (ABOi KT), owing to the mechanism of accommodation. We analyzed the immunodynamics of soluble ABH antigens in allografts from secretor donors and the influence of such immunodynamics on accommodation and subsequen...

BACKGROUND As antigen-specific immunoadsorption (IA) using the Glycosorb®-ABO columns is becoming increasingly popular in ABO-incompatible (ABOi) transplantation, in this study, we retrospectively investigated the efficacy of Glycosorb®-ABO IA in vivo and ex vivo. We also assessed the risk of anti-A/B antibody (ABab) rebound before and after ABOi kidney transplantation. METHODS A protocol for...

BACKGROUND ABO incompatible kidney transplantation (ABOi-KT) is an important approach for overcoming donor shortages. We evaluated the effect of ABOi-KT on living donor KT. METHODS Two nationwide transplantation databases were used. We evaluated the impact of ABOi-KT on overall living donor transplant activity and spousal donation as subgroup analysis. In addition, we compared the clinical ou...

Frequency of Rhesus and ABO blood groups was determined in 3012 mothers. The blood groups in order of frequency were B, O, A and AB. Only 6.2% mothers were rhesus negative. ABO haemolytie disease of the newborn (HDN) accounted for 15.7% and Rh-HDN for 12.7% of 306 neonate admitted with Jaundice. No significant difference was found in two groups when levels of serum bilirubin, exchange transfusi...