Amelogenins bind to GlcNAc of the dentine-enamel matrix proteins (Ravindranath, R. M. H., Moradian-Oldak, J., Fincham, A. G. (1999) J. Biol. Chem. 274, 2464-2471). The hypothesis that amelogenins may interact with the peptides that mimic GlcNAc is tested. GlcNAc-mimicking peptide (SFGSGFGGGY) but not its variants with single amino acid substitution at serine, tyrosine, or phenylalanine residues...

Recent reports have demonstrated beneficial effects of proinsulin C-peptide in the diabetic state, including improvements of kidney and nerve function. To examine the background to these effects, C-peptide binding to cell membranes has been studied by using fluorescence correlation spectroscopy. Measurements of ligand-membrane interactions at single-molecule detection sensitivity in 0.2-fl conf...

Engineered membrane protein pores have demonstrated applications in biotechnology and synthetic biology. For example, engineering to conduct selectively specific ions or small molecules could lead new biosensors. However, the formation of large transmembrane peptide has not been shown previously as target peptides form a complex reaction mixture environment. Here, we assembled pore from 40 amin...

T cell immune responses are driven by the recognition of peptide antigens (T cell epitopes) that are bound to major histocompatibility complex (MHC) molecules. T cell epitope immunogenicity is thus contingent on several events, including appropriate and effective processing of the peptide from its protein source, stable peptide binding to the MHC molecule, and recognition of the MHC-bound pepti...

GRP94, the endoplasmic reticulum Hsp90 paralog, binds a diverse array of peptides, a subset of which are suitable for assembly onto nascent MHC class I molecules. At present, the mechanism, site, and regulation of peptide binding to GRP94 are unknown. Using VSV8, the immunodominant peptide epitope of the vesicular stomatitis virus, and native, purified GRP94, we have investigated GRP94-peptide ...

The SH2 and SH3 domain structural integrity and functionality were confirmed by peptide binding assays. Binding studies using two phosphotyrosine peptides derived from EGFR (FLPVPE{pY}IN-QSVPKR) and Shc (EEPPDHQ{pY}YNDFPGK) for the Grb2 SH2 domain and a SOS1-derived proline-rich peptide (PVPPPVPPRRRPEY) for the SH3 domains were performed using MST 1. Grb2 was fluorescently labeled using Atto488...

In article number 2200236, Zhihong Zhang, Xiang Yu, and co-workers achieve the direct size conversion of erythrocyte-derived MVs (eMVs) via incubation with an ApoA-I mimetic peptide (R4F) capable binding phospholipids. R4F modification endows eMVs SR-B1 receptor lymph node targeting functions, providing excellent drug delivery system for targeted treatment node-associated diseases.

The solution NMR structure is reported for Ca(2+)-loaded S100B bound to a 12-residue peptide, TRTK-12, from the actin capping protein CapZ (alpha1 or alpha2 subunit, residues 265-276: TRTKIDWNKILS). This peptide was discovered by Dimlich and co-workers by screening a bacteriophage random peptide display library, and it matches exactly the consensus S100B binding sequence ((K/R)(L/I)XWXXIL). As ...

preDQ is a software tool for peptide binding prediction to HLA-DQ2 and HLA-DQ8 proteins specially designed developed the European Food Safety Authority. The able identify peptides and/or predict their affinities. used assess risk of novel cause celiac disease. Predictions made by are based on five models assessment reported outputs. using datasets known non-binding proteins. compiled from liter...

Abstract Background Hemagglutinin is a major surface protein in influenza A virus (IAV), and HA2 relative conserved among different IAVs. It will be meaningful to identify broad-spectrum epitopes based on the protein. Results Overlapping peptides of H5N1 IAV A/Mallard/Huadong/S/2005 were synthesized loaded modified silica gel film form microarray, antisera against subtypes IAVs used screen univ...