Transmural heterogeneity of myocardial metabolism and function are present in the left ventricle under normal and ischemic conditions. To determine if endocardial versus epicardial differences of [Ca2`11 are also present, perfused rat heart studies using indo-1 fluorescence as an index of [Ca2"]i were performed in the left ventricular epicardium and endocardium. Hearts were studied during contr...

The effect of neuromedin U (NMU) on rat paraventricular nucleus (PVN) neurons was examined using whole cell patch-clamp recordings. Under current-clamp, 31% of PVN parvocellular neurons (n = 243) were depolarized by 100 nM NMU, but magnocellular neurons were not affected. NMU (10 nM to 1 microM) resulted in increased basal firing rate and depolarization in a dose-dependent manner with an EC50 o...

The effect of neuromedin U (NMU) on rat paraventricular nucleus (PVN) neurons was examined using whole-cell patch-clamp recordings. Under current-clamp, 31% of PVN pavocellular neurons (n = 243) were depolarized by 100 nM NMU, but magnocellular neurons were not affected. NMU (10 nM to 1 μM) resulted in increased basal firing rate and depolarization in a dose-dependent manner with an EC50 of 70 ...

Architectural proteins mediate interactions between distant sequences in the genome. Two well-characterized functions of architectural protein interactions include the tethering of enhancers to promoters and bringing together Polycomb-containing sites to facilitate silencing. The nature of which sequences interact genome-wide appears to be determined by the orientation of the architectural prot...

We show that the mechanism by which chromatin displaying higher-order structure is usually isolated from nuclei involves a transition to an extended nucleosomal arrangement. After being released from nuclei, chromatin must refold in order to produce the typical chromatin fibers observed in solution. For starfish sperm chromatin with a long nucleosome repeat (222 bp), isolated fibers are signifi...

In this paper, CMOS downsizing trend into sub-70 nm node and further below is described. Downscaling of advanced CMOS devices have been significantly accelerated every time by the update of the ITRS roadmap. Now, on one hand, we can expect the realization of even sub-10 nm CMOS devices, but on the other hand, so many difficulties in technology for the downsizing are anticipated. In this paper, ...

We previously have cloned and characterized a retinoid- and fatty acid-binding glycoprotein (RFABG) isolated from the heads of Drosophila melanogaster. The protein is composed of two glycosylated subunits (Mr = >200,000 and 70,000) and is a member of the proapolipophorin gene family. Spectral analysis of purified RFABG revealed an absolute absorbance peak at 405 nm, which is typical for a heme-...

We use the frequency doubled light from an optically trapped lithium niobate particle for non-intrusive scanning near-field optical microscopy. The detected power from this 50-100 nm diameter probe is currently tens of pW and is expected to approach nW with an improved detection system. The current experimental resolution is approximately 0.5 pm, while the ultimate theoretical resolution is 70-...

Ultraviolet (UV) miniature cerium fluoride lasers have been demonstrated using a low cost, frequency-quadrupled microchip Nd:YAG pump laser. The use of miniature laser cavities was shown to significantly improve the laser performance in the low pump power region. We have achieved slope efficiencies up to 70% and pump thresholds as low as 100 nJ. Continuous tuning from 306 nm to 338 nm was achie...