I. Rats were made severely iron-deficient by feeding with Fe-deficient diets, which contained adequate amounts of zinc. 2. Ten days after an oral dose of 250 ,ug %n the Fe-deficient rats had retained 17 yo whereas control rats had retained only 8 yo of the dose. 3. When unlabelled Zn was given intravenously to the Fe-deficient and control rats there was a reduction in retention of the orally ad...

The ultimate goal of protein engineering is to create novel proteins which will adopt predetermined structures, bind specified ligands, and catalyze new reactions. Here we describe the successful introduction of metal-binding activity into a model four helix bundle protein. The designed binding site is tetrahedral and is formed by two Cys and two His ligands on adjacent helices. We have introdu...

High Zn and Cu fecal concentrations impact the environment. Zn and Cu reductions in feed have compromised swine performance. Substituting amino acid chelated (AAC) sources for traditionally employed inorganic salts (IM) may overcome environmental and swine performance problems. A preliminary study demonstrated less 65Zn was deposited in rat feces due to greater AAC absorption compared to IM (P ...

The uptake of colloid-bound Cd, Cr, and Zn by marine plankton (including the diatom Thalassiosira pseudonana, the dinoflagellate Prorocentrum minimum, and the copepod Paracalanus aculeatus) was studied using the radiotracer technique. Natural organic colloids (operationally defined as particles between 1 kDa and 0.2 μm) were isolated from Hong Kong coastal seawater using cross-flow ultrafiltrat...

The release and transfer of zinc from metallothionein (MT) to zinc-depleted sorbitol dehydrogenase (EC 1.1.1.14) in vitro has been used to explore the role of MT in cellular zinc distribution. A 1:1 molar ratio of MT to sorbitol dehydrogenase is required for full reactivation, indicating that only one of the seven zinc atoms of MT is transferred in this process. Reduced glutathione (GSH) and gl...