The ubiquitously expressed Na(+)/H(+) exchanger isoform 1 (NHE1) functions as a major intracellular pH (pH(i)) regulatory mechanism in many cell types, and in some tissues its activity may contribute to ischemic injury. In the present study, cortical astrocyte cultures from wild-type (NHE1(+/+)) and NHE1-deficient (NHE1(-/-)) mice were used to investigate the role of NHE1 in pH(i) recovery and ...

097. 642 | www.jaids.com q 2009 Lippincott Williams & Wilkins Letters to the Editor J Acquir Immune Defic Syndr Volume 51, Number 5, August 15, 2009

Deadline: February 3, 2006 Please direct all submission inquires to Felecia Marsh at 703/642/9090 or [email protected] Denver, Colorado September 17-22, 2006

The purpose of this study was to investigate the mechanisms responsible for ischemia-induced changes in spontaneous electrical activity. An ischemic-like Tyrode solution (pH 6.6) reversibly depolarized the maximum diastolic potential (MDP) and reduced the action potential (AP) overshoot (OS). We used SNARF-1, which is an indicator of intracellular pH (pH(i)), and perforated-patch techniques to ...

Myocardial stretch elicits a biphasic increase in developed force with a first rapid force response and a second slow force response (SFR). The rapid phase is due to an increase in myofilament Ca(2+) responsiveness; the SFR, analyzed here, is ascribed to a progressive increase in Ca(2+) transients. Experiments were performed in cat papillary muscles to further elucidate the signaling pathway un...

Background—Ca overload plays an important role in the pathogenesis of cardioplegic ischemia-reperfusion injury. The standard technique to control Ca overload has been to reduce Ca in the cardioplegic solution (CP). Recent reports suggest that Na/H exchange inhibitors can also prevent Ca overload. We compared 4 crystalloid CPs that might minimize Ca overload in comparison with standard Mg-contai...