A series of tests were conducted to determine if the sensitivity and efficiency of an established rabbit immunoglobulin (IgG)-specific enzyme-linked immunosorbent assay (ELISA) could be improved for detecting a protein mark on insects. Five variations of ELISA were examined for their ability to detect a rabbit IgG mark on the convergent lady beetle, Hippodamia convergens Guérin-Méneville. The c...

Due to poor diagnostic facilities and a lack of medical alertness, allergy to Vespa wasps may be underestimated. Few allergens have been identified from Vespa wasps.Possible native allergen proteins were purified from the wasp venoms (WV) (Vespa magnifica Smith) by gel filtration, ion exchange chromatography, respectively. Their sequences were determined by Edman degradation and cDNA cloning. T...

Actinidin was used to hydrolyse proteins in whey protein isolate (WPI) and milk concentrate (MPC) reduce immunoreactivity of β-lactoglobulin (β-LG) αs1-casein (αs1-CN). Samples were incubated at an enzyme substrate ratio 1:100 (5.21 units actinidin activity g−1 protein) 10 or 60 °C for up 31 5 h, respectively. Protein hydrolysis determined by the degree SDS-PAGE. Antigenicity hydrolysates β-LG ...

OBJECTIVE To investigate the diagnostic value of commercially available BP230 and BP180-NC16a enzyme-linked immunosorbent assays (ELISAs) in routine practice in patients with bullous pemphigoid (BP). DESIGN Single-center retrospective study. SETTING French academic dermatology department. PATIENTS The study population comprised 138 patients, who were admitted from January 1998 through Dec...

The aim of this study is to determine the amount total aflatoxins and aflatoxin B1 (AFB1) in tomato pepper pastes sold farmer’s markets Istanbul evaluate whether levels were within standards regarded as safe (<5 µg kg-1). For purpose, a 64 samples, including 26 pastes, 15 paprika 23 chili analysed using two different detection methods, enzyme-linked immunoabsorbent assay (ELISA) high-performanc...

Methods Cross-sectional retrospective study determining: 1) serum adalimumab (Ada) levels by a capture ELISA (positive > 5 ng/ml) and 2) levels of AAA by a two-site bridging ELISA [positive > 10 Arbitrary Units (AU)/ml]. The clinical activity was assessed by conventional tests and the physician visual analogue scale (ph-VAS) of 0 to 10, the same day that the blood sample was collected. Blood sa...

Anti-NOR90 antibodies are usually found in patients with systemic sclerosis (SSc); however, their clinical relevance remains obscure. We previously developed an enzyme-linked immunosorbent assay (ELISA) for measuring them to investigate the features of anti-NOR90 antibodies. As we additionally obtained antibody-positive serum samples after our previous research, reexamined significance Serum fr...

Background: Anti-cytosolic 5′-nucleotidase 1A (anti-cN1A) antibodies were proposed as a biomarker for the diagnosis of inclusion body myositis (IBM), but conflicting specificity and sensitivity evidence limits its use. Our study aimed to assess diagnostic accuracy anti-cN1A in cohort patients who underwent line immunoassay suspected idiopathic inflammatory myopathies (IIM). We also assessed agr...