background: efficient screening for detection of colorectal cancer (crc) at earlier stages reduces its mortality. the purpose of this study was to investigate expression of carcinoembryonic antigen (cea) and human telomerase reverse transcriptase (htert) mrna in peripheral blood of crc patients and to present strategies for early detection screen test. methods: twenty seven patients in non-meta...

abstract background thalassemia patients that are conventionally treated by a regular transfusion regimen are exposed to blood born viral infections.the aim of this study was to investigate the seroprevalence of hepatitis b virus (hbv), hepatitis c virus (hcv) , and human immunodeficiency virus(hiv) among all multitransfused thalassemia patients in west north of iran. material and methods a ret...

Background: Visceral leishmaniasis (VL) can lead to death in more than 95% of cases if left untreated. Accurate and early diagnosis has an important role in reducing mortality rate of this disease. Objective: To express recombinant H2B antigen from an Iranian isolate of Leishmania Infantum and evaluate its efficacy in the diagnosis of VL. Metho...

DNA coding for the core antigen from hepatitis B Virus (HBcAg) was amplified, cloned and propagated in E. coli. The core protein was expressed in E. coli and the product was readily detected by Western blot. This protein can be used as a diagnostic material in serum screening tests. To increase the level of expression of this antigen in bacteria, two plasmids were constructed in which the gene ...

CAR-T cell immunotherapy involves genetically reprogrammed T-lymphocytes that interact with cancer cells and activate an anti-tumor immune response. This therapy has been approved for clinical use hematological cancers, but new challenges have emerged in the treatment of solid tumors. Some include heterogeneity antigen expression found tumors, including antigen-positive non-tumoral cells, inhib...

alkylhydroperoxide reductase (ahpc, the 26 kda antigen) is one of the abundant antioxidant enzymes in helicobacter pylori and seems to have a good potential for use in development of immunoassays to detect h. pylori infection in clinical specimens. this study aimed to investigate some properties of this antigen by the produced monoclonal antibodies. five established hybridoma cell lines secreti...

prostate-specific antigen (psa) was purified to homogeneity from human seminal plasma by ion-exchange chromatography on a cm-sephadex c-50 and by gel filtration on a sephacryl s-200 column. a single 33-kda protein band appeared in sds-page. high pressure liquid chromatography (hplc) of the purified protein produced a single peak, while isoelectric focusing demonstrated the presence of five diff...