Cytochrome P-450 (P-450) and associated mono-oxygenase activities were estimated in male and female rat brain microsomes. The P-450 concentration in male rat brain was one-tenth the corresponding hepatic levels, which is considerably higher than earlier reports. A distinct sex-related difference was observed in the levels of total P-450 and mono-oxygenase activities known to be mediated by P-45...

Two electrophoretically homogeneous forms of liver microsomal cytochrome P-450 (P-~~OLM), designated P-450~~~ and LM~ by their relative mobilities, were previously isolated from rabbits induced with phenobarbital and P-naphthoflavone, respectively. In the present immunochemical study, rabbits and goats were inoculated with these purified proteins. Rabbits produced antibodies against P-450~~~ bu...

The expression of four cytochrome (cyt.) P-450 ¡soenzymeshas been studied in preneoplastic and neoplastic lesions during the course of nitrosamine-induced hepatocarcinogenesis in the female Wistar rat. Fol lowing exposure to diethylnitrosamine (50 or 100 ppm in the drinking water) for 10 days, animals were taken sequentially, and the livers were analyzed for the evolution of adenosine triphosp...

Incubation of iodosylbenzene and [125I]iodobenzene with cytochrome P-450 (P-450) leads to the formation of [125I]iodosylbenzene (Burka, L.T., Thorsen, A., and Guengerich, F.P. (1980) J. Am. Chem. Soc. 102, 7615-7616), but to date it has not been possible to observe directly the oxidation of organic halides in NADPH-supported P-450 reactions because of the intrinsic instability of haloso compoun...

In vivo administration of the alcohol dehydrogenase inhibitor pyrazole induces a cytochrome P-450 isozyme. The pyrazole-inducible cytochrome P-450 has been purified from rat livers to electrophoretic homogeneity and its biochemical, spectral, and immunological properties characterized. The final preparation had a specific content of 11 nmol of cytochrome P-450/mg of protein. A single band with ...

Two electrophoretically homogeneous forms of liver microsomal cytochrome P-450 (P-~~OLM), designated P-450~~~ and LM~ by their relative mobilities, were previously isolated from rabbits induced with phenobarbital and P-naphthoflavone, respectively. In the present immunochemical study, rabbits and goats were inoculated with these purified proteins. Rabbits produced antibodies against P-450~~~ bu...

Structural planning is a mixture of basics with science in structural analysis, statics, mechanics materials and dynamics to obtain structures that are safe economical their service life. The purpose this study was plan the dimensions building's portal structure can withstand loads safely. This research conducted Karang Asem 1, Kuningan, Setia Budi, South Jakarta. final result be concluded ther...

Drug exposures can lead to an increased expression of specific cytochrome P-450 proteins (P-450s) as well as of phase II drug-metabolizing enzymes that can greatly augment the metabolism and clearance of therapeutic drugs. Animal models have been used to estimate the potential of drugs to induce P-450s in humans, but differences in drug response among species hinder reliable extrapolation of ob...