1. A study was undertaken to investigate the distribution of ascorbic acid between various cellular components of blood, in normal individuals, and its relation to the plasma concentration. Forty-one unsupplemented individuals and sixteen supplemented (2 g/d for 5 d) individuals were studied. 2. Granulocytes, mononuclear leucocytes, platelets and erythrocytes were separated by differential sedi...

The condensation reaction of phenylhydrazine and dinitrophenylhydrazine with 4-acetyl and 4-benzoyl pyrazolone precipitated air-stable acetyldinitrophenylhydrazone Ampp-Dh, benzoylphenylhydrazone Bmpp-Ph and benzoyldinitrophenylhydrazone Bmpp-Dh in their keto imine form; a study inspired by the burning interest for the development of new bioactive materials with novel properties that may become...

The automation of a method for the simultaneous determination of nitrogen oxides, aldehydes and ketones is explained, and its applicability is shown by means of several field tests. All components were sampled together using impingers filled with a solution of 2,4-dinitrophenylhydrazine (DNPH). The corresponding derivatives were separated by HPLC and quantified by UV/Vis-absorbance at 345 nm. M...

Protein oxidation is involved in regulatory physiological events as well as in damage to tissues and is thought to play a key role in the pathophysiology of diseases and in the aging process. Protein-bound carbonyls represent a marker of global protein oxidation, as they are generated by multiple different reactive oxygen species in blood, tissues and cells. Sample preparation and stabilization...

A method of two-step gradient capillary electrochromatography (CEC) was developed to measure 12 carbonyls (aldehydes and ketones) in indoor air samples. The carbonyls were derivatized with 2,4-dinitrophenylhydrazine (DNPH) and then separated by a two-step gradient CEC on a C8 column. Effects of various instrumental conditions on the separation, including buffer concentration, organic modifiers,...

Abstract Beneath glycation, oxidation reactions may take place at cereal proteins during production of malt. The extent oxidative chemical changes malt has not yet been studied. In the present short communication, protein was characterized by determination free thiol groups and degree methionine as well sites that are reactive to covalent modification 2,4-dinitrophenylhydrazine (DNPH, “protein ...