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The purpose of the present investigation was to establish a method for estimating intracellular Ca(2+) concentrations ([Ca(2+)](i)) in isolated rat epitrochlearis muscles. Epitrochlearis muscles excised from 4-wk-old male Sprague-Dawley rats were loaded with a fluorescent Ca(2+) indicator, fura 2-AM, for 60-90 min at 35 degrees C in oxygenated Krebs-Henseleit buffer. After fura 2 loading and su...
Abstract Study question The endometrial microbiome, in addition to other parameters of inflammation (CD138 presence, NK bright fraction presence), may be a complementary marker ongoing local inflammation. Summary answer Presented results the microbiome comparison with CD 138 indicate endometrium, which is caused by dysbiosis. What known already study and assessment its impact on female reproduc...
Abstract Introduction Sickle cell disease (SCD) is a genetic hemoglobinopathy with clinical and epidemiological relevance. Its manifestations directly affect the quality of life affected individuals. There gap in literature regarding approach to aspects related sexual reproductive health people sickle disease, especially female population, given that most available studies evaluated men. The ai...
The use of serologic assays for influenza A virus (IAV) surveillance in wild birds has increased because of the availability of commercial enzyme-linked immunosorbent assays (ELISAs). Recently, an H5-specific blocking ELISA (bELISA) was shown to reliably detect H5-specific antibodies to low- and high-pathogenic H5 viruses in experimentally infected waterfowl. Mute Swans (Cygnus olor) were frequ...
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