Isopenicillin N synthase (IPNS) is a key enzyme responsible for the catalytic conversion of delta-(L-alpha-aminoadipoyl)-L-cysteinyl-D-valine (ACV) to isopenicillin N in the beta-lactam antibiotic biosynthetic pathway. The Aspergillus nidulans IPNS crystal structure implicated amino acid residues tyrosine-189, arginine-279, and serine-281 in the substrate-binding of the valine carboxylate porti...

A series of mutant lac repressor proteins at positions 281 or 282 was isolated for detailed characterization. Although Cys281 modification by sulfhydryl reagents abrogates pH effects on inducer binding and diminishes operator binding (Daly, T. J., Olson, J. S., and Matthews, K. S. (1986) Biochemistry 25, 5468-5474), substitution at this site by alanine, serine, phenylalanine, isoleucine, or met...

Review of Miguel Ángel Pérez Priego, Historia del libro y edición de textos, Universidad Nacional Educación a Distancia (UNED), Madrid, 2018, 281 pp. ISBN: 9788436274769. ISBN electrónico: 9788436273304.

Objectives: Meniscus repair has correspondingly become more popular, with promise of preservation joint mechanics and hopes preventing secondary arthritis. However, it is not clear the optimal rehabilitation protocol, regards to duration limitation range motion weight bearing, following various meniscal tear types. The purpose this study understand current contextual landscape how practitioners...

Recent reports have documented the presence of SV40 large T antigen (T ag) sequences in a number of human tumors and raised the question of whether cellular immunity to T ag is elicited in such individuals. We used HLA-A2.1 transgenic C57BL/6 mice to identify an epitope from T ag recognized by CD8+ CTLs when presented by this human MHC class I molecule. Immunization of HLA-A2.1 transgenic mice ...

Smartphones evolve in a potential learning companion among students. The specific needs of medical education have brought in light, apps dedicated to medical school. Therefore, the impact that arises in academia attracted the focus of scientific world for further research. The aim of this paper is to identify the usage of medical apps among medical students, exploring their usage patterns in or...

OBJECTIVE This study compared barium esophagram with high-resolution esophageal manometry (HRM) results to determine whether esophagram is an adequate screening examination for esophageal motility disorders, a common cause of dysphagia. STUDY DESIGN Case series with chart review. SETTING Tertiary academic medical center. SUBJECTS AND METHODS A retrospective review was completed of 281 pat...