Toxoplasmosis is a zoonosis caused by the protozoan Toxoplasma gondii, and it is found worldwide. To determine whether ungulates are reservoirs of T. gondii in an isolated and remote region of the northeastern Peruvian Amazon, antibodies to T. gondii were determined in 5 species of ungulates by the modified agglutination test (MAT). These animals were hunted by subsistence hunters along the Yav...

BACKGROUND Mating-type loci in yeasts and ascomycotan filamentous fungi (Pezizomycotina) encode master transcriptional factors that play a critical role in sexual development. Genome-wide analyses of mating-type-specification circuits and mating-type target genes are available in Saccharomyces cerevisiae and Schizosaccharomyces pombe; however, no such analyses have been performed in heterothall...

BACKGROUND In the field of vertical jump diagnostics, force plates (FP) are the reference standard. Recently, despite a lack of evidence, jump mats have been used increasingly. Important factors in favor of jumping mats are their low cost and portability. OBJECTIVES This validity study compared the Haynl-Elektronik jump mat (HE jump mat) with the reference standard force plate. MATERIALS AN...

Aims—To compare three commercial screening tests—the PanBio leptospiral IgM enzyme linked immunosorbent assay (ELISA), the Biolisa leptospiral IgM ELISA, and the indirect haemagglutination assay (IHA)—with the microscopic agglutination test (MAT) and two “in house” ELISAs— urease and horseradish peroxidase (HRP)— for the detection of leptospiral antibodies in a local UK and Eire population. Met...

Liver methionine adenosyltransferase (MAT) plays a critical role in the metabolism of methionine converting this amino acid, in the presence of ATP, into S-adenosylmethionine. Here we report that hydrogen peroxide (H2O2), via generation of hydroxyl radical, inactivates liver MAT by reversibly and covalently oxidizing an enzyme site. In vitro studies using pure liver recombinant enzyme and mutan...

BACKGROUND We observed that some patients with clinical leptospirosis supported by positive results of rapid tests were negative for leptospirosis on the basis of our diagnostic gold standard, which involves isolation of Leptospira species from blood culture and/or a positive result of a microscopic agglutination test (MAT). We hypothesized that our reference standard was imperfect and used sta...

EXPERIMENT & RESULTS ・Augemntation of sports instruction using haptic system ・H(*5#16& 5*-& /'(:,;1& /9*C1'6& 0,2#& ;,'152,(-*9& ,-4('+*2,(-& intuitively&0#1-&2#1&/9*C1'6&*'1&(-&2#1&4,19; ・82,+B9*2,(-&B-,2&I8DJ&2(&=1&0('-&=C&2#1&/9*C1'&*-;&*-&,-62'B52,(-& B-,2&I3DJ&4('&2#1&,-62'B52(' ・"9*C1'6&'151,:1&;,'152,(-&,-62'B52,(-6&2#1C&6#(B9;&)(&,-7&=C&0*C& (4&:,='*2,(-6&)1-1'*21;&=C&13 vibration&*52B*...

Aimed at investigating the recovery of a specific mutant allele of the mating type locus (MAT) by switching a defective MAT allele, these experiments provide information bearing on several models proposed for MAT interconversion in bakers yeast, Saccharomyces cerevisiae. Hybrids between heterothallic (ho) cells carrying a mutant MAT a allele, designated mata-2, and MAT alpha ho strains show a h...

Gibberella zeae, a self-fertile, haploid filamentous ascomycete, causes serious epidemics of wheat (Triticum aestivum) head blight worldwide and contaminates grain with trichothecene mycotoxins. Anecdotal evidence dating back to the late 19th century indicates that G. zeae ascospores (sexual spores) are a more important inoculum source than are macroconidia (asexual spores), although the fungus...

The distribution of mating types of Ascochyta rabiei (teleomorph: Didymella rabiei) was studied on isolates collected from chickpea growing areas of Central Anatolia. Mating type assessments of 45 isolates from 6 different provinces were conducted under laboratory conditions. After keeping for 5-6 weeks, the pseudothecial development and ascospore production were observed on chickpea stem piece...