نتایج جستجو برای: 248 polymorphic bands were amplified primers opa
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The wild relatives are important sources of genes for some desirable traits and adaptation to environmental conditions that can be exploited to enrich the cultivated safflower gene pool. In this study, genetic relationship and finding chance of specific markers between three species of the genus Carthamus, including tinctorius, lanatus and oxyacantha were evaluated by 9 ISSR primers. The s...
The aims of this study were to establish a random amplified polymorphic DNA (RAPD) fingerprint database of chloroplast DNA (cpDNA) from different cultivars of Cornus officinalis and to convert RAPD markers to sequence characterized amplified regions (SCAR) markers. A method of extraction was established that was suitable for obtaining cpDNA from samples rapidly dried in silicone; an RAPD finger...
ustilago hordei (pres) lagerh is a fungus that causes covered smut of barley. this disease reduces yield and is a limiting factor in barley fields of iran. one hundred forty four isolates of u. hordei were collected from 36 regions of khuzestan province during 2009 and the genetic diversity of 100 isolates was evaluated by molecular markers. four out of 14 primers were selected on the basis of ...
frequency of somaclonal variation in embryogenic calli and plants of olea europaea l cv.kroneiki derived from somatic embryogenesis were examined by randomly amplified polymorphic dna (rapd) analysis. radicle and cotyledon (proximal part) of mature zygotic embryo were cultured in omc medium supplemented with iba (5 mgl-1) and 2-ip (0.5 mgl-1). somatic embryogenesis was induced in calli of radic...
In this study, 45 Pleurotus isolates with a wide range of geographical origins (in five provinces of Iran: Isfahan, Chaharmahal Bakhtiary, Kohkiloye boyrahmad, Lorestan and Fars) were collected. A random amplified polymorphic DNA polymerase chain reaction (RAPD-PCR) was used to assess the genetic diversity in ten populations of the Pleurotus eryngii complex. The use of ten primers allowed the p...
In the present study, genetic fingerprints of ten species of Zingiberaceae from eastern India were developed using PCR-based markers. 19 RAPD (Rapid Amplified polymorphic DNA), 8 ISSR (Inter Simple Sequence Repeats) and 8 SSR (Simple Sequence Repeats) primers were used to elucidate genetic diversity important for utilization, management and conservation. These primers produced 789 loci, out of ...
Cadmium is a toxic unnecessary element for humans and plants which is easily absorbed by plants. Genetic variation in cadmium accumulation provides an opportunity for breeding programs to select genotypes with low cadmium. Due to the high cost of plant tissue analysis to determine the amount of cadmium, use of markers to make efficient selection of low cadmium accumulating genotypes is an effec...
Abstract Population genetic diversity contributes to the protection and utilization of germplasm resources, especially via breeding. In present study, start codon targeted polymorphism (SCoT) molecular markers were used study 278 individuals from 10 Prunus sibirica L. populations in Inner Mongolia. A total 289 polymorphic bands amplified with 23 SCoT primers, showing a percentage 98.87% an aver...
Field crop landraces are valuable genetic sources. Twenty populations of Persian clover (Trifolium resupinatum L.) collected from different areas of Iran were used in this study. DNA extractions were carried out using minipreparation method with equal amount of leaves from 30 plants of each population. DNA samples from 20 clover populations were evaluated using semi-random (ISJ) markers. Ten pr...
saffron (crocus sativus) is the most valuable and indigenous crop in iran. the stigmas of flower are used as a popular natural flavouring, colouring and medicinal agent. however, the market suffers from frauds in this plant such as mixing with safflower petals due to high profit. identification of these frauds with conventional and biochemical methods is difficult and low sensitive. therefore, ...
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