As more HIV-infected people gain access to antiretroviral therapy (ART), monitoring HIV drug resistance (HIVDR) becomes essential to combat both acquired and transmitted HIVDR. Studies have demonstrated dried blood spots (DBS) are a suitable alternative in HIVDR monitoring using DBS collected on Whatman 903 (W-903). In this study, we sought to evaluate two other commercially available filter pa...

Affinity cleaving proteins have been synthesized based on the DNA-binding domain of the yeast transcriptional activator GCN4 with the DNA cleaving moiety Fe.EDTA attached at the NH2 terminus [Oakley, M. G., & Dervan, P. B. (1990) Science 248, 847]. Cleavage patterns generated by Fe-EDTA-GCN4(226-281) bound to the DNA sites 5'-CTGACTAAT-3' and 5'-ATGACTCTT-3' reveal that the NH2 termini of the G...

226 words Introduction: 737 words Discussion: 1479 words

BACKGROUND We have recently reported that protein phosphate 2A (PP2A) inactivation resulted in increased phosphorylation of the mitogen-activated protein kinase (MAPK) c-Jun N-terminal kinase 1 (JNK1) and glucocorticoid receptors (GR) at Ser(226), thereby reducing GR nuclear translocation and causing corticosteroid insensitivity in severe asthmatics. Protein tyrosine phosphatases (PTPs) are als...

UNLABELLED This study aimed at describing and analyzing tympanometric results obtained with 226 Hz and 1000 Hz probe tones; checking for correlations between tympanometry, otoacoustic emissions and otoscopic examination; describing abnormal results found in the evaluation procedures. METHODS Double-blind and prospective study. Our sample included 70 babies, between 7 days and one month and 13...

Purtx~se: We like to demonstrate glycation of S,-, R,and y-crystallins in the microsectioned bovine lens, by isoelectric focusing using three staining methods. Methods: Pour bovine lenses of 1.185+0.131 years were frozen-sectioned into equa'cor and 11 layers. Watersoluble crvstallins were seuarated bv isoelectric focusing an8 stained: 1. with ?oomassie brilliant Blue (CBB); 2. with the lectin (...

of the antimicrobial anthocyanin red pigment dracorhodin (Fig. 1B), which is isolated in the exudates of the fruit of Daemonorops draco, known as “dragon’s blood” in traditional Chinese medicine and as Kirin-kakketsu in Japanese. It has been used for the treatment of injuries and bleeding. Many flavonoids have been reported to induce apoptosis in human tumor cells, and therefore the cytotoxic e...

Introduction The larynx of the horse is part of the upper airway and serves as a conduit between the pharynx and the trachea, or the wind pipe that takes inspired air to the lungs for gas exchange. The larynx is comprised of a number of cartilages that work together to allow for increased airflow during exercise and to close the trachea during swallowing to prevent foreign material and food par...