نتایج جستجو برای: 2006 using specific primers fsphrfsphf for these forma specialis
تعداد نتایج: 12044096 فیلتر نتایج به سال:
Background and purpose: Molecular detection of antibiotic resistance in clinical strains of M.tuberculosis is of great importance. In this study, we developed a method for rapid detection of mutations resistant to the rifabutin antibiotic resistant gene. Material and methods: In this study 40 clinical isolates of M.tuberculosis including 12 resistant and 28 susceptible isolates to rifabutin we...
abstract- desert truffles are hypogeous ascomyceteous ectomycorrhizal fungi, occurring in arid and semi-arid ecosystems. a pcr-based method was developed for the identification of 3 major desert truffles of iran: terfezia claveryi, tirmania pinoyi and tirmania nivea based on internal transcribed spacers of rdna. two specific pcr primers were designed for t. claveryi, 4 for t. pinoyi, and 2 for ...
A specific polymerase chain reaction (PCR) method was applied for identification of bovine (Bos taurus), ovine (Ovis aries) and caprine (Capra hircus) pure and binary mixtures of raw and heat-processed meats. These meats are used in food industry products and/or for direct consumption of consumers. The mitochondrial DNA was amplified as a template in a PCR reaction by use of specific primers re...
over the past decades a number of approaches have been applied for forecasting mortality. in 1992, a new method for long-run forecast of the level and age pattern of mortality was published by lee and carter. this method was welcomed by many authors so it was extended through a wider class of generalized, parametric and nonlinear model. this model represents one of the most influential recent d...
The wild relatives are important sources of genes for some desirable traits and adaptation to environmental conditions that can be exploited to enrich the cultivated safflower gene pool. In this study, genetic relationship and finding chance of specific markers between three species of the genus Carthamus, including tinctorius, lanatus and oxyacantha were evaluated by 9 ISSR primers. The s...
one very decisive factor in students’ academic destiny is the result they get at final term examinations. because of its importance, both students and teachers are curiously anxious about them. as normally it is the case, these final term tests are prepared hurriedly in short time by teachers which result in students’ dissatisfaction, complaining on how the test was different from their expecta...
one hundred and fifty blood samples were prepared from cattle of a region in isfahan. the extracted dna from blood cells were analyzed by a. centrale (amori strain) specific nested-pcr using primers derived from 16s rrna gene. all blood smears were negative for a. centrale like structures. the results showed that 2 of total 150 blood samples (1.33%) were a. centrale (amori strain) positive by s...
a specific polymerase chain reaction (pcr) method was applied for identification of bovine (bos taurus), ovine (ovis aries) and caprine (capra hircus) pure and binary mixtures of raw and heat-processed meats. these meats are used in food industry products and/or for direct consumption of consumers. the mitochondrial dna was amplified as a template in a pcr reaction by use of specific primers re...
background: splicing by overlap extension (soe) pcr is used to create mutation in the coding sequence of an enzyme in order to study the role of specific residues in protein’s structure and function.objectives: we introduced a nested-soe-pcr (n –soe-pcr) in order to increase the specificity and generating mutations in a gene by soe-pcr. materials and methods: genomic dna from bacillus thermoca...
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