Herein, we present a novel method of asymmetric dimethylarginine (ADMA) and symmetric dimethylarginine (SDMA) determination within biological samples using protein precipitation and LC/MS/MS. Chromatographic separation of ADMA and SDMA was successfully performed using a silica column with optimized elution, or mobile phase, of 10 mM ammonium acetate buffer H2O/methanol/acetonitrile (20/35/45, v...

BACKGROUND Chagas' disease is an endemic tropical affliction found from southern United States to Argentina. The acute phase of this disease is difficult to study in man because the symptoms are non-specific and most cases require no medical assistance. Experimental models have been developed for sequential studies, and intense parasitism in all organs and tissues, including the pancreas, have ...

A bioassay for possible carcinogenicity of technical-grade hexachloroethane was conducted using Osborne-Mendel rats and B6C3F1 mice. Hexachloroethane in corn oil was administered by gavage, at either of two dosages, to groups of 50 male and 50 female animals of each species. The chemical was administered 5 days a week, cyclically for 44 of 78 weeks in rats and continuously for 78 weeks in mice,...

nanotechnology researchers have identified a wide range of nanoparticle applications that may have an important role in medicine and treatment of diseases. due to lack of detailed documentation about the toxicology of zinc oxide (zno) nanoparticles, this study was aimed to evaluate the absorption of zno nanoparticles in hearts of female nmri mice. overall, 20 adult nmri female mice were studied...

1,6-Hexanediamine (HDA) is an aliphatic amine that is produced in large volumes in the United States. HDA is widely used as a corrosion inhibitor in lubricants and as an intermediate in the industrial synthesis of paints, resins, inks, and textiles. Toxicity studies of the dihydrochloride salt of HDA (HDDC) were conducted in male and female Fischer 344/N rats and B6C3F1 mice by the drinking wat...

The purpose of the study was to assess transgenerational intestinal tumorigenic effects of low dose ionizing radiation employing a well-characterized mouse model of human colorectal cancer. Mice (6 to 8 weeks old APC1638N/+ mice; n=20 per study group) were exposed to whole-body 25 cGy x-rays and mated 2 days post-irradiation. Intestinal tumorigenesis in male and female F1 mice from No Parents I...

this study was designed to investigate the role of gabab receptor agents on imipramine-induced antinociception in ligated and non-ligated mice using hot-plate test. the data showed that different doses of morphine (3, 6, and 9 mg/kg) induced a dose-dependent antinociception in ligated and non-ligated mice. however, the opioid response was decreased in ligated animals. intracerebroventricular (i...

The purpose of the study was to assess transgenerational intestinal tumorigenic effects of low dose ionizing radiation employing a well-characterized mouse model of human colorectal cancer. Mice (6 to 8 weeks old APC1638N/+ mice; n=20 per study group) were exposed to whole-body 25 cGy x-rays and mated 2 days post-irradiation. Intestinal tumorigenesis in male and female F1 mice from No Parents I...

BACKGROUND Innumerable fundic gland polyps (FGPs) (that is massive FGP polyposis) may be found in the human stomach. Innumerable fundic gland cysts (FGCs) develop in mice lacking the gene Atp4a (Atp4a((-/-))), that encodes the enzyme ATPase. The aim was to monitor the evolution of FGCs in Atp4a((-/-)) mice. MATERIALS AND METHODS Six pairs (male-female) of Atp4a((-/-)) mice, 8, 12 and 20 month...

background: acceptance of uterus and reaction between endometrium and embryo has an important role for implantation. muc1 an integral membrane mucin is expressed on the apical surface of uterine epithelial cells and could have effects on its receptivity. objective: the aim of this study was to evaluate the changes in muc1 expression of gravid mouse endometrium with and without hyperstimulation ...