The phototrophic bacterium Rhodobacter capsulatus is able to reduce 2,4-dinitrophenol (DNP) to 2-amino-4-nitrophenol enzymatically and thus can grow in the presence of this uncoupler. DNP reduction was switched off by glutamine or ammonium, but this short-term regulation did not take place in a draTG deletion mutant. Nevertheless, the target of DraTG does not seem to be the nitrophenol reductas...

the present work was carried out to evaluate the removal of p-nitrophenol by adsorption onto olive cake based activated carbon having a bet surface area of 672 m²/g. the batch adsorption experimental results indicated that the equilibrium time for nitrophenol adsorption by olive cake-based activated carbon was 120min. the adsorption data was modeled by equilibrium and kinetic models. the pseudo...

A mol of creatine kinase (EC 2.7.3.2), an enzyme with two identical subunits can be titrated very rapidly and stoichiometrically with 2 mol of 2-chloromercuri-4-nitrophenol, an environmentally sensitive chromophoric probe specific for thiols. Chemical modification does not inactivate the enzyme nor can the reaction be prevented by a dead-end quaternary complex of enzyme, magnesium, ADP, and cre...

As a novel approach to characterize the phenolic pollutants of Lake Balaton (Central Europe, western Hungary), 26 endocrine disrupting phenols (chlorophenols, nitrophenols, alkylphenols, triclosan, bisphenol-A) were quantified in dissolved and suspended particulate matter (SPM) phases, alike. Sample collection was performed in the western and eastern basins, at 20 sites in April and October 201...

1. A new and rapid continuous assay of rat liver microsomal UDP-glucuronyltransferase (EC 2.4.1.17) has been developed. It is based on measurement of UDP production from UDP-glucuronate during the glucuronidation reaction; UDP production was continuously measured by coupling it to the conversion of NADH into NAD+ through pyruvate kinase and lactate dehydrogenase. This assay is independent of th...

An environmental actinomycetes, capable of utilizing p-nitrophenol as its sole carbon and nitrogen source, was starved for an 8-week period and showed no reduction in its ability to biodegrade p-nitrophenol. Microscopic examination revealed that starvation of the bacterium resulted in the fragmentation of filaments into individual cells.

Housefly UDP-glucosyltransferase activity towards p-nitrophenol was demonstrated in a system in vitro. The activity is localized in the microsomal fraction, requires UDPglucose, is slightly stimulated by Mg2+ and is activated optimally over a wide range of detergent concentration. Phenobarbital increases the enzyme(s) activity about 3-fold, with p-nitrophenol as substrate, which differs from th...

It has been known for years that the phenolic compounds are able to exert harmful effects toward living organisms including humans due their high toxicity. Living were exposed these as they released into environment waste products from several fast-growing industries. In this regard, tremendous efforts have made by researchers develop sensing methods detection of compounds. Graphene and conduct...