Li-sheng XuGui-zhen GaoWen-gen CaoLiang ZhaoJun ChenNina Bao
L-2-methyltryptophan was prepared from L-serine and 2-methylindole catalyzed by recombinant Escherichia coli with tryptophan synthase activity. In the present research, we used tryptophan synthase (TSase) from recombinant Escherichia coli with pET28a-trpBA plasmid expressing tryptophan synthase from Escherichia coli k-12 MG1655 to produce 2-methyltryptophan, and optimized the conditions for enz...
Journal:
:American journal of physiology. Renal physiology2012
Carlos P VioMariana Quiroz-MunozCatherina A CuevasCarlos CespedesNicholas R Ferreri
Cyclooxygenase-2 (COX-2) is constitutively expressed and highly regulated in the thick ascending limb (TAL). As COX-2 inhibitors (Coxibs) increase COX-2 expression, we tested the hypothesis that a negative feedback mechanism involving PGE(2) EP3 receptors regulates COX-2 expression in the TAL. Sprague-Dawley rats were treated with a Coxib [celecoxib (20 mg·kg(-1)·day(-1)) or rofecoxib (10 mg·kg...