نتایج جستجو برای: 16s rrna hp1

تعداد نتایج: 36763  

Bahram Nasr Esfahani Ensieh Sarikhani Jamshid Faghri, Sharareh Moghim,

Introduction Many studies have shown epidemiological links between strains isolated in tap water, and those isolated from patients. Molecular methods linked to PCR are more reliable and faster for identification of             non- tuberculous mycobacteria(NTM). In this study molecular methods were used for identification and typing of NTM. Materials and Methods Five hundred ml of 85 water ...

Journal: :Applied and environmental microbiology 2008
Xu Li Eberhard Morgenroth Lutgarde Raskin

The potential of a solution-based hybridization assay using peptide nucleic acid (PNA) molecular beacon (MB) probes to quantify 16S rRNA of specific populations in RNA extracts of environmental samples was evaluated by designing PNA MB probes for the genera Dechloromonas and Dechlorosoma. In a kinetic study with 16S rRNA from pure cultures, the hybridization of PNA MB to target 16S rRNA exhibit...

Journal: :Clinical microbiology reviews 2004
Jill E Clarridge

The traditional identification of bacteria on the basis of phenotypic characteristics is generally not as accurate as identification based on genotypic methods. Comparison of the bacterial 16S rRNA gene sequence has emerged as a preferred genetic technique. 16S rRNA gene sequence analysis can better identify poorly described, rarely isolated, or phenotypically aberrant strains, can be routinely...

Journal: :Antimicrobial agents and chemotherapy 2003
P Pfister M Risch D E Brodersen E C Böttger

Hygromycin B is an aminoglycoside antibiotic active against prokaryotic and eukaryotic ribosomes. Ribosomal alterations in bacteria conferring resistance to hygromycin B have not been described, prompting us to use a single rRNA allelic derivative of the gram-positive bacterium Mycobacterium smegmatis for investigation of the molecular mechanisms involved in ribosomal resistance to hygromycin B...

Journal: :Proceedings of the National Academy of Sciences of the United States of America 1978
J Brosius M L Palmer P J Kennedy H F Noller

The complete nucleotide sequence of the 16S RNA gene from the rrnB cistron of Escherichia coli has been determined by using three rapid DNA sequencing methods. Nearly all of the structure has been confirmed by two to six independent sequence determinations on both DNA strands. The length of the 16S rRNA chain inferred from the DNA sequence is 1541 nucleotides, in close agreement with previous e...

2014
Hiroshi Mori Fumito Maruyama Hiromi Kato Atsushi Toyoda Ayumi Dozono Yoshiyuki Ohtsubo Yuji Nagata Asao Fujiyama Masataka Tsuda Ken Kurokawa

The deep sequencing of 16S rRNA genes amplified by universal primers has revolutionized our understanding of microbial communities by allowing the characterization of the diversity of the uncultured majority. However, some universal primers also amplify eukaryotic rRNA genes, leading to a decrease in the efficiency of sequencing of prokaryotic 16S rRNA genes with possible mischaracterization of...

Journal: :Journal of bacteriology 1988
Y Suzuki Y Ono A Nagata T Yamada

The number of rRNA genes in Streptomyces lividans was examined by Southern hybridization. Randomly labeled 23 and 16S rRNAs were hybridized with BamHI, BglII, PstI, SalI, or XhoI digests of S. lividans TK21 DNA. BamHi, BglII, SalI and XhoI digests yielded six radioactive bands each for the 23 and 16S rRNAs, whereas PstI digests gave one band for the 23S rRNA and one high-intensity band and six ...

Journal: :Environmental microbiology 2002
Bryce P Nelson Mark R Liles Kendra B Frederick Robert M Corn Robert M Goodman

In this paper, we describe the detection of bacterial cell-extracted 16S ribosomal RNA (rRNA) using an emerging technology, surface plasmon resonance (SPR) imaging of DNA arrays. Surface plasmon resonance enables detection of molecular interactions on surfaces in response to changes in the index of refraction, therefore eliminating the need for a fluorescent or radioactive label. A variation of...

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