Protein crystallographers are well aware of the trap crystallizing E. coli proteins instead macromolecule interest if heterologous recombinant protein expression in was part experimental pipeline. Among well-known culprits YodA metal-binding lipocalin (25 kDa) and YadF carbonic anhydrase (a tetramer 25 kDa subunits). We report a novel crystal form another such culprit, HPII catalase, which is t...

Recently, a new biomarker of mosquito bite has been developed using human antibody response to salivary protein. One the proteins that tested is protein 34-kDa which refined Nterm-34 kDa peptide. This study aimed characterize peptide as potential antigen asses Dengue virus transmission risk. The sequence was analyzed several software predict characteristic BLASTP from Vectorbase and NCBI wa use...

Complement C3 is the central component of the human complement system. It is ~186 kDa in size, consisting of an α-chain (~110 kDa) and a β-chain (~75 kDa) that are connected by cysteine bridges. C3 in its native form is inactive. Cleavage of C3 into C3b (~177 kDa) and C3a (~9 kDa) is a crucial step in the complement activation cascade, which can be initiated by one or more of the three distinct...

Abstract Introduction Transthyretin (TTR) is a homotetrameric 55-KDa plasma protein that transports thyroxin and retinol complexed to retinol-binding (RBP). TTR misfolding aggregation can lead the extracellular deposition of amyloid (ATTR) representing one most frequent forms amyloidosis in elderly. Aim this study develop native electrophoretic method characterize circulating samples ATTR patie...

Heparin-binding epidermal growth factor-like growth factor (HB-EGF) is a member of the epidermal growth factor family and has a variety of physiological and pathological functions. Modulation of HB-EGF activity might have a therapeutic potential in the oncology area. We explored the therapeutic possibilities by characterizing the in vitro biological activity of anti-HB-EGF monoclonal antibody Y...

Bile acids are involved in the modulation of various metabolic processes facilitating biliary excretion endogenous and exogenous cholesterol. The objective this study was to determine glycocholic acid binding capacity (BC) chicken blood hydrolysates using an optimized RP-HPLC methodology. Samples were hydrolysed a combination five different enzymes. Alcalase Protamex presented highest BC, with ...

Cross-flow ultrafiltration (UF) membrane with two different configurations; single (10 kDa and 5 kDa) two-stage (10/5 in fractionating microalgae protein hydrolysate (MPH) were studied to obtain a low molecular weight of peptide. The effect flow rate, trans-membrane pressure (TMP), pH MPH evaluated based on permeate flux peptide transmission. results showed that, for UF membrane, optimum operat...

Chronic allograft dysfunction (CAD) remains the major cause of renal transplant loss and characterized by interstitial fibrosis and tubular atrophy (IFTA). MicroRNAs (miRNAs) are implicated in many biological processes as well as innate and adaptive immune responses. We aimed to investigate whether CAD with IFTA is associated with differential expression of miR-142-5p, miR-142-3p and miR-211 wi...