OBJECTIVES Functional characterization of a mutant Candida albicans drug resistance protein (Cdr1p) by overexpression in Saccharomyces cerevisiae. METHODS We overexpressed green fluorescent protein-tagged Cdr1p in S. cerevisiae AD1-8u- host and introduced a point mutation to substitute T1351 with F in Cdr1p. The cells expressing T1351F mutant Cdr1p were analysed for their functional activity ...

References 1 Keller, M. and Zengler, K. (2004) Tapping into microbial diversity. Nat. Rev. Microbiol. 2, 141–150 2 DeLuca, T.H. et al. (2002) Quantifying nitrogen-fixation in feather moss carpets of boreal forests. Nature 419, 917–920 5 Cardinale, B.J. et al. (2006) Effects of biodiversity on the functioning o trophic groups and ecosystems. Nature 443, 989–992 6 Tylianakis, J.M. et al. (2008) G...

Abstract—Fruit juices play important roles in human health as being a key part of nutrition. Juice and nectar are two categories of drinks with so many variations for consumers, regardless of age, lifestyle and taste preferences, which they can find their favorites. Juices contain 100% pulp when pulp content of ‘nectar’ changes between 25%-50%. In this study, potassium (K), magnesium (Mg), and ...

Boo-Chai (1966) reported a case of successful closure of a cleft lip at approximately 390 A.D. in China, although the surgeon's name is not mentioned. Yperman (1295-1351) was a Flemish surgeon who appears to have written the first fully documented description of cleft lip and its surgical repair. He closed the freshened borders of the cleft lip with a triangular needle armed with a twisted wax ...

We investigate the hybrid exotic meson with J = 1−+ within the framework of an AdS/QCD model. Introducing a holographic field dual to the operator for hybrid exotic meson, we obtain the eigen-value equation for its mass. Fixing all free parameters by QCD observables such as the ρ-meson mass, we predict the masses of the hybrid exotic meson. The results turn out to be 1476MeV for the ground stat...

We surveyed 1351 residents who lived near six U.S. Department of Energy (DOE) former nuclear weapon sites using random digit dialing and stratified random sampling in order to determine which risks most worried them and the predictors of their worries. Respondents’ greatest concerns were threats to their drinking water, transportation accidents, and worker exposures. The strongest predictors of...

As a first step toward understanding how rare variants contribute to risk for complex diseases, we sequenced 15,585 human protein-coding genes to an average median depth of 111× in 2440 individuals of European (n = 1351) and African (n = 1088) ancestry. We identified over 500,000 single-nucleotide variants (SNVs), the majority of which were rare (86% with a minor allele frequency less than 0.5%...