snail is one of the worst causes of food allergy. thus, the aim of this study was to identify the major and minor allergens of the local marine snail ( cerithidea obtusa ) and subsequently to investigate the impacts of heat treatment on the ige-binding activity of snail allergens. proteins from raw and heat-treated snails (boiled, roasted and fried) were extracted and then resolved by sodium do...

Ligand-induced regulation of the rat -opioid receptor (rKOR) was investigated in human embryonic kidney 293 cells stably expressing the FLAG-tagged rKOR. Incubation of rKOR cells with naltrexone for 24 h increased the Bmax 3-fold, with no change in the affinity of [H]diprenorphine. Two immunoreactive receptor species were present in cell lysates: naltrexone treatment caused a 3-fold increase in...

  Partially fed (4 -5 days) H .a. anatolicum (Iranian isolate) female adult ticks were used for preparation of antigens of midgut which are, the gut supernate antigen (GS), gut membrane antigen (GM), triton extracted gut membrane antigen ( TX-GM ) and sodium dodecyl sulphate treated Triton extracted gut membrane antigen (SDS-TX-GM). The New Zealand white rabbits were immunized with GS, GM, TX- ...

The present study was carried out to study the national haemorrhagic septicaemia vaccine strains at their molecular level. The vaccine is bivalent contain Pasteurella multocida serotype E:2 and B:2. The vaccine strains were obtained from Central Veterinary Research Laboratory, Department of Biological Product, Soba. Culture characteristic and colonial morphology was ascertained in common labora...

A 44-kDa major outer membrane protein of the human granulocytic ehrlichiosis (HGE) agent is an immunodominant antigen in human infection. A gene encoding this protein was cloned and sequenced. Southern blot results revealed the existence of multigenes homologous to the P44 gene in the genome of the HGE agent. The recombinant 44-kDa protein (rP44) was expressed by using expression vector pET30a....

The use of nanomaterials for enhancement the antibacterial activity different compounds is a new approach contributing to efforts overcoming emerging antibiotics resistance. This study explores combined nanodiamonds and snail peptides against Brevibacillus laterosporus BT271. Two peptide/protein fractions from Cornu aspersum mucus were used – with molecular weight (MW) below 10 kDa MW above 30 ...

Background: Diabetic nephropathy in patients on insulin is characterized by glucose-induced inhibition of matrix-degrading enzymes is believed to contribute the matrix accumulation. Study Design: Study tried to find out the renal tubular proteins that may detect earlier phase of renal complication in diabetes. Material and Methods: 42 female patients and 27 male patients using insulin (18-20 un...