In this study we demonstrate that the different substrate recognition properties of bacterial and human AP endonucleases might be used to quantify and localize apurinic (AP) sites formed in DNA in vivo. By using a model oligonucleotide containing a single AP site modified with methoxyamine (MX), we show that endonuclease III and IV of E. coli are able to cleave the alkoxyamine-adducted site whe...

A gas chromatographic method using a capillary column with electron capture detection was developed for the simultaneous determination of 3 nitromusk fragrance ingredients: musk ambrette (MA), musk xylol (MX), and musk ketone (MK), in fragrance products. The accuracy of the method was determined by recovery of each nitromusk from fortified fragrance products at 3 different concentrations. Recov...

Great genetic variability among teleost immunomes, with gene losses and expansions of central adaptive and innate components, has been discovered through genome sequencing over the last few years. Here, we demonstrate that the innate Myxovirus resistance gene (Mx) is lost from the ancestor of Gadiformes and the closely related Stylephorus chordatus, thus predating the loss of Major Histocompati...

A solid dispersion of Meloxicam (MX), a poorly soluble, non steroidal anti-inflammatory drug, and Gelucire 50/13 was prepared by spray drying. Spherical microparticles were yielded with smooth surfaces as observed by scanning electron microscopy. According to differential scanning calorimetry and powder X-ray diffractometry analysis, MX was transformed from the crystalline state to the amorphou...

Proof of proposition. We know quite generally that Supp s is a closed set (indeed, for any sheaf of abelian groups on any topological space; see Exercise II.1.14 of [1]). On a Noetherian scheme, it is then a finite union of irreducible components, and we simply need to show that each generic point of each component is an associated point of F . But this is clear, since if x is such a generic po...

BACKGROUND Individualizing mean arterial blood pressure (MAP) based on cerebral blood flow (CBF) autoregulation monitoring during cardiopulmonary bypass (CPB) holds promise as a strategy to optimize organ perfusion. The purpose of this study was to evaluate the accuracy of cerebral autoregulation monitoring using microcirculatory flow measured with innovative ultrasound-tagged near-infrared spe...

Fix a morphism of schemes f : X → S which is flat, proper, and “fiber-by-fiber semi-stable”. Let IV LS be the functor on the category of log schemes over S which to any T associates the isomorphism classes of pairs (MX , f), where MX is a log structure on X×S T and f : pr∗ 2MT →MX is a morphism of log structures making (X×S T ,MX)→ T a log smooth, integral, and vertical morphism. The main resul...

BACKGROUND Coccidioides immitis and C. posadasii cause coccidioidomycosis, a disease that is endemic to North and South America, but for Central America, the incidence of coccidioidomycosis has not been clearly established. Several studies suggest genetic variability in these fungi; however, little definitive information has been discovered about the variability of Coccidioides fungi in Mexico ...

Three-dimensional (3D) HPLC methods are useful tools for the selective analysis of biomolecules in complicated real-world matrices, however, columns applicable second dimension still limited. In present study, two types mixed-mode (Singularity MX-102 and 103) were newly designed/developed focusing on separation N-protected chiral amino acids. The retention profiles evaluated using 10 proteinoge...