BACKGROUND Metastasis-associated in colon cancer 1 (MACC1) is demonstrated to be up-regulated in several types of cancer, and can serve as biomarker for cancer invasion and metastasis. To investigate the relations between MACC1 and biological processes of ovarian cancer, MACC1 specific small hairpin RNA (shRNA) expression plasmids were used to investigate the effects of MACC1 inhibition on ovar...

Objectives: This study assessed the antitumoral activity of MEK inhibitor trametinib (TMT212) and ERK1/2 SCH772984, alone in combination with CDK4/6 ribociclib (LEE011) human neuroendocrine tumor (NET) cell lines vitro. Methods: Human NET BON1, QGP-1, NCI-H727 were treated or ribociclib, to assess proliferation, cycle distribution, protein signaling using flow cytometry, Western blot assays, re...

Purpose: To investigate the role of centrosomal protein55 (CEP55) in melanoma. 
 Methods: The CEP55 expression human melanoma cells and normal melanocytes were determined by quantitative reverse transcription-polymerase chain reaction (qRT-PCR) western blot. Cell proliferation was evaluated colony formation CCK8 assays, while cell cycle assessed flow cytometry. Transwell assay used to migr...

Background Zinc finger protein 259 (ZNF259) is known to play essential roles in embryonic development and cell cycle regulation. However, its expression pattern and clinicopathological relevance remain unclear. Materials and methods A total of 114 lung cancer specimens were collected. The ZNF259 expression was measured between the lung cancer tissues and the adjacent normal lung tissues by im...

To explore how cytohesin-1 (CYTH-1) small interfering RNA (siRNA) influences the insulin-like growth factor receptor (IGFR)-associated signal transduction in prostate cancer, we transfected human prostate cancer PC-3 cell lines with liposome-encapsulated CYTH-1 siRNA in serum-free medium and exposed the cells to 100 nM IGF-1. The mRNA and protein levels of the signal molecules involved in the I...

A diet rich in fruits and vegetables, and a grape-derived compound, resveratrol, have been linked to a reduced incidence of colon cancer. In vitro and in vivo, resveratrol suppresses Wnt signaling, a pathway constitutively activated in over 85 % of colon cancers.Thirty participants were placed on a low resveratrol diet and subsequently allocated to one of three groups ingesting 1/3-to-1 lb (0.1...

Mesenchymal stem cells (MSCs) have the potential to be the source for cell-based therapies. However, MSCs can undergo malignant transformation in a tumor microenvironment where a high level of interleukin (IL)-6 is present. In this study, we investigated the role of IL-6 and signal transducer and activator of transcription 3 (STAT3) signaling in malignant transformation of MSCs. Rat MSCs were i...