OBJECTIVE To screen and characterize germline variants for E-cadherin (CDH1) in non-hereditary gastric cancer (GC) patients and in subjects at risk of GC. METHODS 59 GCs, 59 first degree relatives (FDRs) of GC, 20 autoimmune metaplastic atrophic gastritis (AMAGs) and 52 blood donors (BDs) were analyzed for CDH1 by direct sequencing, structural modelling and bioinformatics. Functional impact o...

Bone morphogenic protein (BMP) signalling contributes towards maintenance of pluripotency and favours mesodermal over neural fates upon differentiation, but the mechanisms by which BMP controls differentiation are not well understood. We report that BMP regulates differentiation by blocking downregulation of Cdh1, an event that accompanies the earliest stages of neural and mesodermal differenti...

The anaphase-promoting complex/cyclosome (APC/C) regulates sister chromatid segregation and the exit from mitosis. Selection of most APC/C substrates is controlled by coactivator subunits (either Cdc20 or Cdh1) that interact with substrate destruction motifs--predominantly the destruction (D) box and KEN box degrons. How coactivators recognize D box degrons and how this is inhibited by APC/C re...

BACKGROUND Most gastric cancer (GC) is sporadic and seem to be mostly related to a cumulative effect of multiple environmental factors. Although the actual importance of genetic factors has not yet been fully documented, GC with familial aggregation has been found to have an incidence of 10% to 30%. MATERIALS AND METHODS Genetic factors contribute to the well-known autosomal dominant syndrome...

  Absract   Gastric cancer is the fourth most common cancer and the second leading cause of   cancer death. Most cases are sporadic and only 10% of patients, show familial clustering. Among these patients, 1 to 3 % have hereditary diffuse gastric cancer   (HDGC), which is autosomal-dominant and present in younger ages. Mutations in Ecadherin gene CDH1 has been identified in 30 to 50% of patient...

In this study, we examined the effect of a combination surface topographies (fiber alignments) and different stiffness poly(L-lactic acid) (PLLA) substrates on direct relationship between anti-cancer drug (CDDP) sensitivity for MDA-MB-231 cells mesenchymal properties under both normal hypoxic conditions. addition, studied induction epithelial–mesenchymal transition (EMT). The CDDP treatment hyp...

Hereditary diffuse gastric cancer (HDGC) is an inherited form of gastric cancer that carries a poor prognosis. Most HDGCs are caused by an autosomal dominant genetic mutation in the CDH1 gene, which carries a 70%-80% lifetime risk of gastric cancer. Given its submucosal origin, endoscopic surveillance is an unreliable means of early detection, and prophylactic gastrectomy is recommended for CDH...

In ECAD application areas there is a real need for electrical circuit modelling and analy sis tools at an intermediate level between analogue and state based simulators Numerical tools do not capture the abstractions valued by engineers and logical functional models do not maintain su cient a nities with the electrical properties of a system This paper examines the nature of this problem and th...

BACKGROUND Approximately one third of the hereditary diffuse gastric cancer (HDGC) families carry germline mutations in the E-cadherin gene (CDH1). Risk prediction in members of families with this rare but deadly cancer could be improved by the identification of additional HDGC genes in non-CDH1 families. METHODS Affected individuals from 22 CDH1 mutation-negative families were screened for g...