this study was conducted to optimize a highly efficient mrna transfection into dendritic cells (dc) derived from esophageal squamous cell carcinoma (escc) patients. applying an electroporation technique, in vitro synthesized green fluorescent protein (gfp) mrna was transfected as an indicator into the dcs derived from a healthy donor. flow cytometry revealed 84.9% transfection efficiency for dc...

Background: T helper 1 and T helper 17 cells play important roles in immunity against foreign invaders. Differentiation of these Th subsets is affected by state of maturation and cytokines that are produced by dendritic cells (DCs). Curdlan is a linear (1→3)-β- glucan and has shown activity against tumors and infectious agents. Objective: This study aims to investigate whether curdlan plays its...

Abstract Dendritic cells (DCs) are essential players in the anti-tumor immunity, which often found dysfunctional tumor microenvironment. Impairing DC antigen processing and presentation, subverts T cell priming, is one of primary mechanisms by escape immune surveillance. However, cellular factors regulating presentation DCs how render DCs’ priming capacity not fully understood. We revealed a po...

Constructing a more effective value at risk (VaR) prediction model has long been goal in financial management. In this paper, we propose novel parametric approach and provide standard paradigm to demonstrate the modeling. We establish dynamic conditional score (DCS) based on high-frequency data generalized distribution (GD), namely, GD-DCS model, improve forecasts of daily VaR. The assumes that...

the antioxidant activities of tbhq, bht and tocopherol mixtures at different concentrations (0, 100, 200, 400 mg/kg) have been evaluated by the application of differential scanning calorimetry (dsc) and rancimat apparatus. rancimat apparatus that measures the secondary oxidation products was set at 110 ⁰c with an air flow of 18-20 l/h to measure the induction period (ip) of the samples. the dsc...

in many practical distribution networks, managers face significant uncertainties in demand, local price of building facilities, transportation cost, and macro and microeconomic parameters. this paper addresses design of distribution networks in a supply chain system which optimizes the performance of distribution networks subject to required service level. this service level, which is considere...

With the advent of big data era, centers (DCs) related energy use accounts for approximately 3% global electric power consumption. As augmentation data-processing performance and thermal density in DCs, its will undoubtedly continuously burst. Meanwhile, potential safety hazards, caused by IT equipment local overheating, threaten safe operation restrict further development DCs. Thus, efficient ...