Nuclei isolated from cauliflower mosaic virus (CaMV) infected turnip leaves contain subgenomic CaMV DNA species in addition to the genome length CaMV DNA. These subgenomic CaMV DNA species are present as covalently closed circles (form I), relaxed circles (form II) and linear (form III) molecules. The subgenomic form I DNA species range in size from about 10% of genome length to nearly genome l...

Cauliflower mosaic virus (CaMV) replicated in protoplasts and in inoculated leaves of the non-host, cotton (Gossypium hirsutum, L.). Protoplasts prepared from suspension-cultured cotton cells were infected by incubation with liposome-encapsulated CaMV virions. During a 1-week culture period the amount of CaMV nucleic acid as detected by nucleic acid hybridization in the protoplasts increased si...

artemisia annua is an annual herb native to asia most probably china produce one of the most important sesquiterpene namely artemisinin which has antimalarial and anticancer activity. three transcription factors belonging to wrky family, two transcription factors belonging to myb family and two enzymes dbr2 and opr3 were isolated from a.  annua leaves and first inserted into pjet1.2 vector then...

Abstract Diseases caused by bacteria are an important and widespread constraint, occurring in almost all crops, including vegetables, pulses, cereals, ornamentals, fruit forages. Although several strategies have been developed to obtain disease resistance plants using genetic engineering, few studies effectively demonstrated the control of bacterial diseases. It has previously reported that a g...

Miraculin, a taste modifier, is protein that was first isolated from miracle fruit (Richadella dulcifica). It can change sour into sweet when acids are consumed, although it does not elicit response. Miraculin may have the potential in industry as substitute for sugars and artificial sweeteners. Since plant has low productivity, mass production of miraculin limited. Transgenic hairy root cultur...

Translation of the polycistronic 35S RNA of CaMV (cauliflower mosaic virus) occurs via a reinitiation mechanism, which requires TAV (transactivator/viroplasmin). To allow translation reinitiation of the major open reading frames on the polycistronic RNA, TAV interacts with the host translational machinery via eIF3 (eukaryotic initiation factor 3) and the 60S ribosome. Accumulation of TAV and eI...

The P6 protein of Cauliflower mosaic virus (CaMV) transactivates translation of the CaMV 35S polycistronic pregenomic RNA and its spliced versions, and thus allows synthesis of a complete set of viral proteins. Previous studies have shown that P6 interacts with plant L18 and L24 ribosomal proteins and initiation factor eIF3, and it has been proposed that these interactions are involved in the r...

trr14 is a novel protein important in trehalose (α-d-glucosyl-[1,1]-α-d-glucopyranoside) signaling in arabidopsis. in this research, we provided evidences to demonstrate that trr14 plays role in arabidopsis responses to salt and drought stress. the transgenic arabidopsis plants over-expressing trr14 under the control of camv 35s promoter were generated. transformed lines showed higher transcrip...