The effect of biotic stress induced by viral infection (Potato virus Y, strain NTN and O) on NADP-malic enzyme (EC 1.1.1.40) in tobacco plants (Nicotiana tabacum L., cv. Petit Havana, SR1) was tested at the transcriptional, translational and activity level. The increase of enzyme activity in infected leaves was correlated with the increased amount of expressed protein and with mRNA of cytosolic...

Abstract Potato virus Y (PVY) causes major yield losses in potato ( Solanum tuberosum L.) crops worldwide. Over the past few decades, two new PVY strains (PVY N−Wi/N:O and NTN ) have become prevalent widely distributed In Kenya, there is limited information on prevalence distribution of cultivars grown farmer’s fields. This a limiting factor making decisions for setting up basis control. study ...

One of the most severe symptoms caused by compatible plant-virus interactions is systemic necrosis, which shares common attributes with the hypersensitive response to incompatible pathogens. Although several studies have identified viral symptom determinants responsible for systemic necrosis, mechanistic models of how they contribute to necrosis in infected plants remain scarce. Here, we examin...

The dN/dS ratio between nonsynonymous and synonymous substitution rates has been used extensively to identify codon positions involved in adaptive processes. However, the accuracy of this approach has been questioned, and very few studies have attempted to validate experimentally its predictions. Using the coat protein (CP) of Potato virus Y (PVY; genus Potyvirus, family Potyviridae) as a case ...

Each season, crops face simultaneous or successive multiple infections. Yet cultivar resistance is often bred against one pest at a time, and screened under single This raises the question of expression mono-infections when exposed to We tackled this by measuring level quantitative potato cultivars either Globodera pallida Phytophthora infestans infections, using virus Y (PVY) Rhizoctonia solan...

In comparison to single infections, co-infection of Nicotiana benthamiana with Potato virus X (PVX) and Potato virus Y (PVY) or Plum pox virus (PPV), resulted in increased systemic symptoms (synergism in pathology). Previous studies have shown that virus infections affected the accumulation of various microRNAs (miRNAs) and miRNA target genes. Our studies revealed that double infection by PVX a...

Experiments were conducted to study the plant growth and tuber yield of the seed potato collected from PVY and PLRV infected plants. The seed potatoes were graded as under size (<28 mm), A grade ( 28-40 mm), B grade (41-55 mm) and over size (>55 mm) which were planted following RCBD in the field. Third generation seed potatoes of Diamant variety were used. The incidence of viruses was confirmed...

A highly sensitive RT-PCR protocol able to detect potato virus Y (PVY) in pooled sample units (tubers) was developed. PVY-specific primers selected in the coat protein gene were found to amplify a 359 bp fragment from diluted crude extract of infected tubers. For the detection of the amplification products, a colorimetric detection procedure in microtiter plates was established. The amplicons a...

potato virus y (pvy) is one of the most important pathogenic agents that reduce the yield and quality of tobacco (nicotiana tabacum l.). dna-based molecular markers are valuable tools for identifying germplasm in gene banks, assessing kinship ties, and doing marker-assisted selection (mas) for resistance to pests, diseases, and some crop traits of interest. this work presents the results of ass...